Sun Dan, Wang Wei, Fan Qian, Wang Xinyi, Xu Xinyan, Jiang Weiye, Zhao Liang, Li Hong, Fu Zhifang, Zhao Lei, Jiao Hongmei
Department of Geriatrics, Peking University First Hospital, Beijing 100034, China.
Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Nutrients. 2025 Jan 29;17(3):502. doi: 10.3390/nu17030502.
BACKGROUND/OBJECTIVES: This study aimed to evaluate the renal protective effects of black rice anthocyanins (BRAs) against renal injury in mice induced by D-galactose (D-gal).
The renal aging mouse model was established by thirteen consecutive weeks of subcutaneous injections of D-gal. The serum levels of urea nitrogen (BUN), creatinine (CRE), uric acid (UA), antioxidant enzymes (e.g., GSH-Px and SOD), and total antioxidant capacity (T-AOC), as well as the contents of inflammatory factors (IL-1β, IL-6, and TNF-α) in kidney tissues were evaluated. Additionally, the relative expression of the NQO1, HO-1, IKKβ, NF-kBp65, and TLR4 proteins was examined.
BRA treatment significantly reduced serum levels of BUN, and CRE increased the concentrations of antioxidant enzymes and total antioxidant capacity in renal tissues, and reduced the levels of inflammatory factors. Furthermore, BRAs restored the relative expression of the NQO1, HO-1, IKKβ, NF-kBp65, and TLR4 proteins to normal levels and promoted the recovery of the renal tissue architecture.
It was demonstrated that BRAs could potentially prevent and protect against kidney injury by modulating the Nrf2 and NF-κB signaling pathways, attenuating oxidative stress and inflammatory responses, and modulating the gut microflora. These findings provide a scientific basis for the application of BRAs as a natural bioactive substance in the field of nephroprotection, especially against the renal degeneration that accompanies the aging process.
背景/目的：本研究旨在评估黑米花青素（BRAs）对D-半乳糖（D-gal）诱导的小鼠肾损伤的肾脏保护作用。
通过连续13周皮下注射D-半乳糖建立肾脏衰老小鼠模型。评估血清尿素氮（BUN）、肌酐（CRE）、尿酸（UA）、抗氧化酶（如谷胱甘肽过氧化物酶和超氧化物歧化酶）和总抗氧化能力（T-AOC）水平，以及肾组织中炎症因子（白细胞介素-1β、白细胞介素-6和肿瘤坏死因子-α）的含量。此外，检测NQO1、HO-1、IKKβ、NF-κBp65和TLR4蛋白的相对表达。
BRA治疗显著降低血清BUN和CRE水平，提高肾组织中抗氧化酶浓度和总抗氧化能力，降低炎症因子水平。此外，BRAs将NQO1、HO-1、IKKβ、NF-κBp65和TLR4蛋白的相对表达恢复到正常水平，并促进肾组织结构的恢复。
结果表明，BRAs可能通过调节Nrf2和NF-κB信号通路、减轻氧化应激和炎症反应以及调节肠道微生物群来预防和保护肾脏损伤。这些发现为BRAs作为一种天然生物活性物质在肾脏保护领域的应用提供了科学依据，尤其是针对伴随衰老过程的肾脏退化。
