Milutinović Milica, Ćujić Nikolić Nada, Cvetković Tatjana, Šavikin Katarina, Djordjević Ivana, Janković Veličković Ljubinka, Randjelović Milica, Miladinović Bojana, Branković Suzana, Kitić Dušanka
Department of Pharmacy, Faculty of Medicine, University of Niš, Bul. Dr Zorana Đinđića 81, 18000 Niš, Serbia.
Institute of Medicinal Plants Research, Dr Josif Pančić, Tadeuša Koščuška 1, 11000 Beograd, Serbia.
Plants (Basel). 2024 Nov 7;13(22):3136. doi: 10.3390/plants13223136.
The study aimed to investigate the protective effects of chokeberry fruit products and by-products against cisplatin-induced acute nephrotoxicity in rats. Potential mechanisms involving oxidative stress and inflammatory responses were examined through biochemical and histopathological analyses of kidney tissue. Chokeberry waste, along with the whole fruit extract and juice, was evaluated as a potential raw material for pharmaceutical use. The chemical composition of chokeberry juice and extracts was analyzed using spectrophotometry and HPLC. Rats were treated with chokeberry preparations via intragastric tube for ten days, with a single intraperitoneal dose of cisplatin (8 mg/kg BW) administered on the third day. Post-sacrifice, plasma samples were analyzed for biochemical nephrotoxicity markers, oxidative stress, and inflammatory markers. Kidneys were removed for histopathological and biochemical analysis. Cisplatin-induced acute nephrotoxicity was confirmed by elevated plasma creatinine and blood urea nitrogen levels. Additionally, lipid peroxidation was significantly elevated, while reduced glutathione and catalase activity were significantly reduced. Pro-inflammatory mediators IL-1, TNF-, and IL-6 levels were significantly increased in the cisplatin group. Treatment with chokeberry extracts and juice significantly mitigated these nephrotoxic effects, as confirmed by histopathological examination and biochemical marker analysis. Notably, the waste extract demonstrated greater efficacy than the whole fruit extract, likely due to its higher concentration of polyphenolic compounds, especially anthocyanins. These results highlight the potential of chokeberry as a therapeutic and preventive agent for kidney protection, emphasizing the value of by-products rich in biologically active compounds.
该研究旨在调查黑果腺肋花楸果实产品及其副产品对顺铂诱导的大鼠急性肾毒性的保护作用。通过对肾脏组织进行生化和组织病理学分析，研究了涉及氧化应激和炎症反应的潜在机制。对黑果腺肋花楸废料以及整个果实提取物和果汁进行了评估，将其作为潜在的药用原料。使用分光光度法和高效液相色谱法分析了黑果腺肋花楸果汁和提取物的化学成分。大鼠通过胃管接受黑果腺肋花楸制剂治疗十天，在第三天腹腔注射单次剂量的顺铂（8 mg/kg体重）。处死后，分析血浆样本中的生化肾毒性标志物、氧化应激和炎症标志物。取出肾脏进行组织病理学和生化分析。血浆肌酐和血尿素氮水平升高证实了顺铂诱导的急性肾毒性。此外，脂质过氧化显著升高，而还原型谷胱甘肽和过氧化氢酶活性显著降低。顺铂组中促炎介质IL-1、TNF-α和IL-6水平显著升高。组织病理学检查和生化标志物分析证实，用黑果腺肋花楸提取物和果汁治疗可显著减轻这些肾毒性作用。值得注意的是，废料提取物显示出比整个果实提取物更高的疗效，这可能是由于其多酚化合物尤其是花青素的浓度更高。这些结果突出了黑果腺肋花楸作为肾脏保护治疗和预防剂的潜力，强调了富含生物活性化合物的副产品的价值。
