Rehman Shamsur, Rashid Arif, Manzoor Muhammad Aamir, Li Lingling, Sun Weibo, Riaz Muhammad Waheed, Li Dawei, Zhuge Qiang
Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics and Biotechnology, College of Biology and the Environment, Nanjing Forestry University, Ministry of Education, Nanjing, China.
State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, China.
Front Genet. 2022 Feb 8;12:784878. doi: 10.3389/fgene.2021.784878. eCollection 2021.
Superoxide dismutase (SOD) is an important enzyme that serves as the first line of defense in the plant antioxidant system and removes reactive oxygen species (ROS) under adverse conditions. The SOD protein family is widely distributed in the plant kingdom and plays a significant role in plant growth and development. However, the comprehensive analysis of the gene family has not been conducted in Cucurbitaceae. Subsequently, 43 genes were identified from Cucurbitaceae species [ (watermelon), (zucchini), (cucumber), (bottle gourd), (melon)]. According to evolutionary analysis, genes were divided into eight subfamilies (I, II, III, IV, V, VI, VII, VIII). The gene structure analysis exhibited that the gene family had comparatively preserved exon/intron assembly and motif as well. Phylogenetic and structural analysis revealed the functional divergence of Cucurbitaceae gene family. Furthermore, microRNAs 6 miRNAs were predicted targeting 3 genes. Gene ontology annotation outcomes confirm the role of under different stress stimuli, cellular oxidant detoxification processes, metal ion binding activities, SOD activity, and different cellular components. Promoter regions of the family revealed that most -elements were involved in plant development, stress response, and plant hormones. Evaluation of the gene expression showed that most genes were expressed in different tissues (root, flower, fruit, stem, and leaf). Finally, the expression profiles of eight genes analyzed by qRT-PCR suggested that these genetic reserves responded to drought, saline, heat, and cold stress. These findings laid the foundation for further study of the role of the gene family in Cucurbitaceae. Also, they provided the potential for its use in the genetic improvement of Cucurbitaceae.
超氧化物歧化酶（SOD）是一种重要的酶，在植物抗氧化系统中充当第一道防线，并在不利条件下清除活性氧（ROS）。SOD蛋白家族广泛分布于植物界，在植物生长发育中发挥着重要作用。然而，葫芦科尚未对该基因家族进行全面分析。随后，从葫芦科物种[西瓜、西葫芦、黄瓜、瓠瓜、甜瓜]中鉴定出43个基因。根据进化分析，这些基因被分为八个亚家族（I、II、III、IV、V、VI、VII、VIII）。基因结构分析表明，该基因家族的外显子/内含子组装和基序也相对保守。系统发育和结构分析揭示了葫芦科基因家族的功能差异。此外，预测有6个microRNA靶向3个基因。基因本体注释结果证实了该基因在不同应激刺激、细胞氧化解毒过程、金属离子结合活性、SOD活性以及不同细胞成分中的作用。该基因家族的启动子区域显示，大多数元件参与植物发育、应激反应和植物激素。基因表达评估表明，大多数基因在不同组织（根、花、果实、茎和叶）中表达。最后，通过qRT-PCR分析的8个基因的表达谱表明，这些基因储备对干旱、盐渍、热和冷胁迫有反应。这些发现为进一步研究葫芦科基因家族的作用奠定了基础。此外，它们为其在葫芦科遗传改良中的应用提供了潜力。
