Congthai Walainud, Phosriran Chutchawan, Chou Socheata, Onsanoi Kanyarat, Gosalawit Chotika, Cheng Kuan-Chen, Jantama Kaemwich
Metabolic Engineering Research Unit, School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Suranaree Subdistrict, Muang District, Nakhon Ratchasima 30000, Thailand.
Institute of Food Science and Technology, College of Bioresources and Agriculture, National Taiwan University, Taipei 10617, Taiwan.
Int J Mol Sci. 2025 Jan 24;26(3):982. doi: 10.3390/ijms26030982.
Succinic acid is applied in many chemical industries in which it can be produced through microbial fermentation using lignocellulosic biomasses. Mixed-waste office paper (MWOP) containing lignocellulosic fibers is enormously generated globally. MWOP is recycled into toilet paper and cardboard, but the recovery process is costly. The reuse of MWOP to alternatively produce succinic acid is highly attractive. In this study, pretreatment of MWOPs with 1% (/) HSO at 121 °C for 20 min was found to be optimal. The optimal conditions for the enzymatic hydrolysis of HSO-pretreated MWOP (AP-MWOP) were at 50 °C, with cellulase loading at 80 PCU/g AP-MWOP. This resulted in the highest glucose (22.46 ± 0.15 g/L) and xylose (5.11 ± 0.32 g/L). Succinic acid production via separate hydrolysis and fermentation (SHF) by KJ122 reached 28.19 ± 0.98 g/L (productivity of 1.17 ± 0.04 g/L/h). For simultaneous saccharification and fermentation (SSF), succinic acid was produced at 24.58 ± 2.32 g/L (productivity of 0.82 ± 0.07 g/L/h). Finally, succinic acid at 51.38 ± 4.05 g/L with yield and productivity of 0.75 ± 0.05 g/g and 1.07 ± 0.08 g/L/h was achieved via fed-batch pre-saccharified SSF. This study not only offers means to reuse MWOP for producing succinic acid but also provides insights for exploiting other wastes to high-value succinic acid, supporting environmental sustainability and zero-waste society.
琥珀酸应用于许多化学工业中，在这些工业中它可以通过利用木质纤维素生物质进行微生物发酵来生产。全球大量产生含有木质纤维素纤维的混合办公废纸（MWOP）。MWOP被回收制成卫生纸和硬纸板，但回收过程成本高昂。将MWOP用于替代生产琥珀酸极具吸引力。在本研究中，发现用1%（/）硫酸在121℃下对MWOP进行20分钟的预处理是最佳的。硫酸预处理的MWOP（AP-MWOP）酶水解的最佳条件是在50℃，纤维素酶负载量为80 PCU/g AP-MWOP。这产生了最高的葡萄糖（22.46±0.15 g/L）和木糖（5.11±0.32 g/L）。通过KJ122进行的分步水解和发酵（SHF）生产琥珀酸达到28.19±0.98 g/L（生产率为1.17±0.04 g/L/h）。对于同步糖化发酵（SSF），琥珀酸产量为24.58±2.32 g/L（生产率为0.82±0.07 g/L/h）。最后，通过补料分批预糖化SSF实现了琥珀酸产量为51.38±4.05 g/L，产率和生产率分别为0.75±0.05 g/g和1.07±0.08 g/L/h。本研究不仅提供了将MWOP用于生产琥珀酸的再利用方法，还为开发其他废物生产高价值琥珀酸提供了见解，支持环境可持续性和零废物社会。
