Choi Hee-Young, Kim Hyun-Joo, Lee Ju-Youn, Joo Ji-Young
Department of Periodontology, Dental and Life Science Institute, School of Dentistry, Pusan National University, Yangsan 50612, Republic of Korea.
Department of Periodontology, Dental Research Institute, Pusan National University Dental Hospital, Yangsan 50612, Republic of Korea.
J Clin Med. 2025 Jan 29;14(3):896. doi: 10.3390/jcm14030896.
: As non-thermal atmospheric pressure plasma (NTP) is known to have advantages in application in the medical field, we consider its applicability to periodontitis, a representative chronic inflammatory disease. The purpose of this study was to evaluate the effect of NTP in inhibiting the progression of periodontitis in a rat model when additionally used in scaling and root planing (SRP). : To induce experimental periodontitis in 20 rats, ligatures were placed in the maxillary second molar and lipopolysaccharide from was injected around the teeth. Then, NTP treatment was performed for 2 or 5 min, together with scaling and root planing (SRP). To evaluate alveolar bone loss, micro-computed tomography (micro-CT) analysis and hematoxylin-eosin (H-E) staining were performed. Tartrate-resistant acid phosphatase (TRAP) analysis was performed to compare the number of osteoclasts, while immunohistochemistry (IHC) analysis was performed to determine the expression levels of receptor activator of nuclear factor-B ligand (RANKL) and osteoprotegerin (OPG). Enzyme-linked immunosorbent assay (ELISA) analysis was performed for the detection of cytokines (TNF-α, IL-1β, and IL-10) in tissues and sera. : When SRP was combined with NTP, alveolar bone loss was decreased, the number of osteoclasts and RANKL expression were decreased, OPG expression was increased, and pro-inflammatory cytokine (TNF-α and IL-1β) levels were significantly decreased. Compared with the NTP treatment for 2 min, when treated for 5 min, less alveolar bone loss, fewer osteoclasts, a lower RANKL expression level, and a higher OPG expression level were observed. : This study evaluated the adjunctive treatment effect of NTP in periodontitis-induced rats. Based on the results of this study, we suggest that supplemental NTP treatment may be a good option for non-surgical periodontal treatment; however, further studies are needed to elucidate the mechanism through which NTP suppresses periodontal inflammation.
由于非热大气压等离子体（NTP）在医学领域的应用具有优势，我们考虑其对牙周炎（一种典型的慢性炎症性疾病）的适用性。本研究的目的是评估在龈下刮治和根面平整（SRP）基础上额外使用NTP对大鼠牙周炎进展的抑制作用。：为在20只大鼠中诱导实验性牙周炎，在上颌第二磨牙放置结扎丝，并在牙齿周围注射脂多糖。然后，进行2分钟或5分钟的NTP治疗，同时进行龈下刮治和根面平整（SRP）。为评估牙槽骨吸收，进行了显微计算机断层扫描（micro-CT）分析和苏木精-伊红（H-E）染色。进行抗酒石酸酸性磷酸酶（TRAP）分析以比较破骨细胞数量，同时进行免疫组织化学（IHC）分析以确定核因子-κB受体活化因子配体（RANKL）和骨保护素（OPG）的表达水平。进行酶联免疫吸附测定（ELISA）分析以检测组织和血清中的细胞因子（TNF-α、IL-1β和IL-10）。：当SRP与NTP联合使用时，牙槽骨吸收减少，破骨细胞数量和RANKL表达降低，OPG表达增加，促炎细胞因子（TNF-α和IL-1β）水平显著降低。与2分钟的NTP治疗相比，5分钟治疗时观察到更少的牙槽骨吸收、更少的破骨细胞、更低的RANKL表达水平和更高的OPG表达水平。：本研究评估了NTP对牙周炎诱导大鼠的辅助治疗效果。基于本研究结果，我们认为补充NTP治疗可能是非手术牙周治疗的一个良好选择；然而，需要进一步研究以阐明NTP抑制牙周炎症的机制。
