Garcia-Quinto Ernestina, Guisan Jose M, Fernandez-Lorente Gloria
Laboratory of Microbiology and Food Biocatalysis, Institute of Food Science Research (CIAL, CSIC-UAM), Nicolás Cabrera, 9, UAM Campus, Cantoblanco, 28049 Madrid, Spain.
Department of Biocatalysis, Institute of Catalysis and Petrochemistry (ICP, CSIC), Marie Curie, 2, UAM Campus, Cantoblanco, 28049 Madrid, Spain.
Molecules. 2025 Feb 6;30(3):728. doi: 10.3390/molecules30030728.
Recent studies have shown that DHA supplementation in the form of phospholipids effectively increases DHA levels in the brain, including DHA lysophospholipids. This research explores a method to produce DHA lysophosphatidylcholine (DHA-LPC) using lipases and phospholipases immobilized on Immobeads-C18 with maximal enzyme loading. The esterification of glycerophosphatidylcholine (GPC) and DHA was studied with ionic liquids as alternatives to traditional solvents, with 1-methyl-3-octylimidazolium tetrafluoroborate (MOIM-BF) providing the highest yield due to its ability to increase the solubility of GPC. The reaction parameters were modified to establish a molar ratio of GPC to DHA of 1/10. A maximum DHA-LPC yield of 80% was achieved in 48 h, with a formation rate of 20.06 (mg/mL.h) × g. The Quara LowP biocatalyst (QlowP-C18) maintained 100% activity during the first three cycles and produced 788 mg of DHA lysophospholipid. The use of 50% MOIM-BF improved the stability of the biocatalyst, and NMR confirmed that the product was the sn1-DHA-LPC isomer.
最近的研究表明，以磷脂形式补充DHA可有效提高大脑中的DHA水平，包括DHA溶血磷脂。本研究探索了一种使用固定在Immobeads-C18上的脂肪酶和磷脂酶以最大酶负载量生产DHA溶血磷脂酰胆碱（DHA-LPC）的方法。研究了甘油磷脂酰胆碱（GPC）和DHA的酯化反应，使用离子液体替代传统溶剂，其中1-甲基-3-辛基咪唑四氟硼酸盐（MOIM-BF）由于能够提高GPC的溶解度而提供了最高的产率。修改反应参数以建立GPC与DHA的摩尔比为1/10。在48小时内实现了80%的最大DHA-LPC产率，生成速率为20.06（mg/mL·h）×g。Quara LowP生物催化剂（QlowP-C18）在前三个循环中保持100%的活性，并产生了788mg的DHA溶血磷脂。使用50%的MOIM-BF提高了生物催化剂的稳定性，核磁共振证实产物为sn1-DHA-LPC异构体。
