College of Food Science, Northeast Agricultural University.
College of Grain Science and Technology, Shenyang Normal University.
J Oleo Sci. 2021 Apr 2;70(4):531-540. doi: 10.5650/jos.ess20268. Epub 2021 Mar 10.
In this study, Novozym 435-catalyzed interesterification of ethyl ferulate (EF) with phosphatidylcholine (PC) in a two-phase system consisting of an ionic liquid (IL) and toluene was optimized to prepare feruloylated lysophospholipids (FLPs). Optimum conditions for the interesterification process were found to be [Bmim][TfN]/toluene ratio of 1:1 (v/v), solvent volume of 4 mL, molecular sieves (4 Å) concentration of 80 mg/mL, reaction temperature of 55°C, substrate molar ratio of 5:1 (PC/EF), Novozym 435 concentration of 50 mg/mL. Under these conditions, two FLPs products (1-FLP and 2-FLP) with total conversion rate of 50.79% were obtained. Because the formation of 1-FLP was significantly higher than 2-FLP, 1-FLP was purified and characterized by LC-MS and NMR. In addition, 1-FLP showed DPPH scavenging activity comparable with those of EF and BHT. Therefore, this study provides a good method for transformation of ferulic acid to improve its solubility and promote its application as functional ingredient in the food and pharmaceutical industries.
在这项研究中，Novozym 435 催化的酯交换反应，在两相体系中用乙基阿魏酸酯（EF）和磷脂酰胆碱（PC），离子液体（IL）和甲苯。优化条件制备阿魏酰溶血磷脂（FLPs）。最适反应条件为[Bmim][TfN] /甲苯体积比为 1：1（v/v），溶剂体积为 4 毫升，4 Å 分子筛浓度为 80 毫克/毫升，反应温度为 55°C，底物摩尔比为 5：1（PC/EF），Novozym 435 浓度为 50 毫克/毫升。在这些条件下，两种 FLPs 产物（1-FLP 和 2-FLP）的总转化率为 50.79%。由于 1-FLP 的形成明显高于 2-FLP，因此通过 LC-MS 和 NMR 对 1-FLP 进行了纯化和表征。此外，1-FLP 具有与 EF 和 BHT 相当的 DPPH 清除活性。因此，本研究为阿魏酸的转化提供了一种很好的方法，以提高其溶解度，并促进其在食品和制药工业中作为功能性成分的应用。
