Xue Lili, Dai Jingqi, Fu Ruyu, Wu Nana, Yu Jiaxuan, Dong Jie, Yang Tao, Fan Jinping
College of Horticulture and Landscape, Northeast Agricultural University, Harbin 150030, China.
Plants (Basel). 2025 Jan 22;14(3):323. doi: 10.3390/plants14030323.
Lily () is a globally known perennial herbaceous bulbous flower, popular for its large, colourful flowers and high economic and ornamental value. However, pollen generation is a severe issue that reduces the cosmetic value of lilies. In this study, the MADS transcription factor LiAG1 was isolated and identified from the 'Ice Pink Queen', a male-sterile variety obtained through several years of hybridisation in our laboratory. qRT-PCR revealed that expression was greater in lily anthers, especially during the half-opening stage. The transient expression in tobacco demonstrated that LiAG1 was located in the nucleus. In the 'Ice Pink Queen' lily, suppression of using TRV-VIGS (tobacco-rattle-virus-mediated virus-induced gene silencing) resulted in the disappearance of most of the tapetum layer and the absence of the microsporangia. Overexpression of in transgenic Arabidopsis and tobacco resulted in narrower and more involute leaves, plant dwarfing, earlier blooming, and better pollen viability. Overall, our results suggested that might play an important role in flower development, especially anther development, of 'Ice Pink Queen'.
百合是一种全球闻名的多年生草本球根花卉，因其花朵大、色彩艳丽且具有很高的经济和观赏价值而备受欢迎。然而，花粉生成是一个严重问题，会降低百合的观赏价值。在本研究中，从我们实验室经过数年杂交获得的雄性不育品种“冰粉女王”中分离并鉴定出MADS转录因子LiAG1。qRT-PCR显示，LiAG1在百合花药中的表达量更高，尤其是在半开放阶段。在烟草中的瞬时表达表明LiAG1定位于细胞核。在“冰粉女王”百合中，使用TRV-VIGS（烟草脆裂病毒介导的病毒诱导基因沉默）抑制LiAG1导致大部分绒毡层消失且没有小孢子囊。在转基因拟南芥和烟草中过表达LiAG1导致叶片变窄且更内卷、植株矮化、开花提前以及花粉活力提高。总体而言，我们的结果表明LiAG1可能在“冰粉女王”的花发育尤其是花药发育中起重要作用。
