College of Landscape Architecture, Zhejiang A&F University, Hangzhou 311300, China.
College of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, China.
Int J Mol Sci. 2023 Dec 28;25(1):406. doi: 10.3390/ijms25010406.
belongs to the Malvaceae family, and is a plant with medicinal, edible, and greening values. MADS-box transcription factor is a large family of regulatory factors involved in a variety of biological processes in plants. Here, we performed a genome-wide characterization of MADS-box proteins in and investigated gene structure, phylogenetics, -acting elements, three-dimensional structure, gene expression, and protein interaction to identify candidate MADS-box genes that mediate petal developmental regulation in . A total of 163 candidate MADS-box genes were found and classified into type I (Mα, Mβ, and Mγ) and type II (MIKC and M). Analysis of -acting elements in the promoter region showed that most elements were correlated to plant hormones. The analysis of nine expressions of two different cultivars showed that they were differentially expressed between two type flowers. The analysis of protein interaction networks also indicated that MADS proteins played a crucial role in floral organ identification, inflorescence and fruit development, and flowering time. This research is the first to analyze the MADS-box family of and provides an important reference for further study of the biological functions of the MADS-box, especially in flower organ development.
属于锦葵科，是一种具有药用、食用和绿化价值的植物。MADS 盒转录因子是一个涉及植物多种生物学过程的调控因子大家族。在这里，我们对 进行了 MADS 盒蛋白的全基因组特征分析，并研究了基因结构、系统发育、顺式作用元件、三维结构、基因表达和蛋白质相互作用，以鉴定候选 MADS 盒基因，这些基因可能介导 在花瓣发育调控中的作用。共发现 163 个候选 MADS 盒基因，分为 I 型（Mα、Mβ 和 Mγ）和 II 型（MIKC 和 M）。启动子区顺式作用元件分析表明，大多数元件与植物激素相关。对两个不同 品种的 9 个基因表达分析表明，它们在两种类型的花之间存在差异表达。蛋白质相互作用网络分析也表明，MADS 蛋白在花器官识别、花序和果实发育以及开花时间中起着关键作用。这项研究首次对 进行了 MADS 盒家族分析，为进一步研究 MADS 盒的生物学功能，特别是在花器官发育方面，提供了重要参考。
