在无菌大鼠体内和体外测定肠道细菌的β-葡萄糖醛酸酶活性。
beta-Glucuronidase activities of intestinal bacteria determined both in vitro and in vivo in gnotobiotic rats.
作者信息
Gadelle D, Raibaud P, Sacquet E
出版信息
Appl Environ Microbiol. 1985 Mar;49(3):682-5. doi: 10.1128/aem.49.3.682-685.1985.
Abstract
The beta-glucuronidase activities of bacterial strains isolated from the rat intestinal tract were studied both in vitro in culture media and in vivo in the intestinal contents of gnotobiotic rats. Only 50 of 407 strains tested were found to be positive in vitro. They belonged to the three genera Clostridium, Peptostreptococcus, and Staphylococcus. The in vitro-negative strains were also negative in vivo. The beta-glucuronidase activities of the beta-glucuronidase activities of the positive strains were generally greater in vivo than in vitro. The highest in vivo activities were found in the intact bacterial cells and in the soluble fractions prepared from disrupted pellets. There was a discrepancy between the activities obtained from both conventional and gnotobiotic rats harboring selected positive strains, suggesting that the main beta-glucuronidase-positive strains have not yet been isolated from the intestines of conventional rats.
摘要
对从大鼠肠道分离出的细菌菌株的β-葡萄糖醛酸酶活性进行了研究，研究包括在培养基中进行的体外研究以及在无菌大鼠肠道内容物中进行的体内研究。在测试的407株菌株中，仅50株在体外呈阳性。它们属于梭菌属、消化链球菌属和葡萄球菌属这三个属。体外呈阴性的菌株在体内也呈阴性。阳性菌株的β-葡萄糖醛酸酶活性通常在体内比在体外更高。在完整的细菌细胞以及从破碎沉淀制备的可溶性部分中发现了最高的体内活性。在携带选定阳性菌株的常规大鼠和无菌大鼠中获得的活性之间存在差异，这表明尚未从常规大鼠的肠道中分离出主要的β-葡萄糖醛酸酶阳性菌株。
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