Hofflin J M, Remington J S
Arch Intern Med. 1985 May;145(5):925-6.
A man with acquired immunodeficiency syndrome and multiple opportunistic infections developed central nervous system signs, and results of computed tomography suggested brain abscess. Histologic study and culture of material obtained from the brain biopsy specimen revealed Aspergillus. The clot from a peripheral venous blood sample obtained at the time of biopsy was processed and cultured on a monolayer of L cells. Five days later, Toxoplasma tachyzoites were identified in the cell culture. We bring this case to the attention of the medical community to point out that inoculation of tissue cell cultures as for viral isolation may aid in the diagnosis of Toxoplasma infection in these and other immunocompromised patients.
一名患有获得性免疫缺陷综合征并伴有多种机会性感染的男子出现了中枢神经系统症状，计算机断层扫描结果提示脑脓肿。对脑活检标本进行组织学研究和培养后发现曲霉菌。对活检时采集的外周静脉血样本的凝块进行处理，并在L细胞单层上进行培养。五天后，在细胞培养物中鉴定出弓形虫速殖子。我们提请医学界关注该病例，以指出像用于病毒分离那样接种组织细胞培养物可能有助于诊断这些以及其他免疫功能低下患者的弓形虫感染。
