Wu Ranfeng, Zhang Yongpeng, Teng Jiongjiong, Zhang Qiang, Zhang Cheng
School of Computer Science and Technology, Dalian University of Technology, Dalian 116024, China.
School of Control and Computer Engineering, North China Electric Power University, Beijing 100096, China.
ACS Nano. 2025 Feb 25;19(7):6918-6928. doi: 10.1021/acsnano.4c13882. Epub 2025 Feb 13.
DNA storage has become an attractive alternative to long-term, stable digital data storage because of its high storage density and strong stability. Recently, numerous efforts have been made to develop DNA data access methods to improve the efficiency and accuracy of molecular data reading. However, most current data access methods were achieved by well-developed polymerase chain reaction (PCR) and DNA hybridization, which lack the exploration of dynamic and programmable operations for data access. Here, we propose a programmable DNA data access strategy in which the nanoparticle folders are controlled by DNAzyme circuits to achieve specific information manipulation. We experimentally demonstrate three kinds of circuit programs that access specific information in YES, AND, and OR logic manner. In addition, the selective information access was performed by using a DNAzyme circuit to obtain the target information from a DNA data pool. Importantly, we have extended the circuit-controlled framework to multiple manipulation modes, demonstrating four manipulations on two AuNP folders to access different information on demand. The programmable access strategy provides a paradigm for integrated DNA computing and storage systems and has more applications in the fields of molecular computation and DNA data storage.
由于其高存储密度和强稳定性，DNA存储已成为长期、稳定的数字数据存储的一种有吸引力的替代方案。最近，人们为开发DNA数据访问方法做出了许多努力，以提高分子数据读取的效率和准确性。然而，目前大多数数据访问方法是通过成熟的聚合酶链反应（PCR）和DNA杂交实现的，这些方法缺乏对数据访问的动态和可编程操作的探索。在此，我们提出了一种可编程DNA数据访问策略，其中纳米颗粒折叠体由DNAzyme电路控制，以实现特定信息的操纵。我们通过实验展示了三种以“是”、“与”和“或”逻辑方式访问特定信息的电路程序。此外，通过使用DNAzyme电路从DNA数据库中获取目标信息，实现了选择性信息访问。重要的是，我们将电路控制框架扩展到多种操纵模式，展示了对两个金纳米颗粒折叠体的四种操纵，以按需访问不同信息。这种可编程访问策略为集成DNA计算和存储系统提供了一个范例，在分子计算和DNA数据存储领域有更多应用。
