Key Laboratory of Advanced Design and Intelligent Computing, Dalian University, Ministry of Education, Dalian 116622, China.
School of Computer Science and Technology, Dalian University of Technology, Dalian 116024, China.
Molecules. 2019 Nov 15;24(22):4134. doi: 10.3390/molecules24224134.
Recently, DNA molecules have been widely used to construct advanced logic devices due to their unique properties, such as a simple structure and predictable behavior. In fact, there are still many challenges in the process of building logic circuits. Among them, the scalability of the logic circuit and the elimination of the crosstalk of the cascade circuit have become the focus of research. Inspired by biological allosteric regulation, we developed a controllable molecular logic circuit strategy based on the activity of DNAzyme. The E6 DNAzyme sequence was temporarily blocked by hairpin DNA and activated under appropriate input trigger conditions. Using a substrate with ribonucleobase (rA) modification as the detection strand, a series of binary basic logic gates (YES, AND, and INHIBIT) were implemented on the computational component platform. At the same time, we demonstrate a parallel demultiplexer and two multi-level cascade circuits (YES-YES and YES-Three input AND (YES-TAND)). In addition, the leakage of the cascade process was reduced by exploring factors such as concentration and DNA structure. The proposed DNAzyme activity regulation strategy provides great potential for the expansion of logic circuits in the future.
最近，由于 DNA 分子具有独特的性质，如简单的结构和可预测的行为，因此被广泛用于构建先进的逻辑器件。事实上，在构建逻辑电路的过程中仍然存在许多挑战。其中，逻辑电路的可扩展性和级联电路的串扰消除已成为研究的焦点。受生物变构调节的启发，我们开发了一种基于 DNAzyme 活性的可控分子逻辑电路策略。发夹 DNA 暂时阻断 E6 DNAzyme 序列，并在适当的输入触发条件下被激活。使用带有核糖核苷酸（rA）修饰的底物作为检测链，在计算元件平台上实现了一系列二进制基本逻辑门（YES、AND 和 INHIBIT）。同时，我们展示了一个并行多路分解器和两个多级级联电路（YES-YES 和 YES-Three input AND (YES-TAND)）。此外，通过探索浓度和 DNA 结构等因素，减少了级联过程中的泄漏。所提出的 DNAzyme 活性调节策略为未来逻辑电路的扩展提供了巨大的潜力。
