Ma Mengni, Xu Ding, Chen Runzhou, Shang Junzhong, Ning Guogui
National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan, 430070, China.
The Institute of Flowers Research, Huazhong Agricultural University, Wuhan, 430070, China.
Mol Biol Rep. 2025 Feb 13;52(1):227. doi: 10.1007/s11033-025-10337-1.
Glutathione S-transferases (GSTs) play crucial roles in various physiological processes, including plant development, endogenous metabolism, stress tolerance, and xenobiotic detoxification. However, the comparative analysis of the GST gene family in Rosaceae species and their expression patterns in roses remains unclear.
In this study, a total of 348 GSTs were identified in six Rosaceae species, including 78 in rose (Rosa chinensis), 53 in strawberry (Fragaria vesca), 69 in peach (Prunus persica), 59 in Chinese plum (Prunus mume), 43 in apple (Malus domestica), and 46 in pear (Pyrus communis). These GST proteins were classified into nine subfamilies. Chromosome localization, collinearity analysis, and gene duplication relationships revealed that tandem repeats are the main driving force for the amplification of GST genes in the Rosoideae and Amygdaloideae. Gene Ontology (GO) and cis-element analysis suggested that GST genes may play a significant role in a variety of biological processes and respond to various abiotic stresses. qRT-PCR analysis showed that five GST genes, mainly expressed in the flower tissue of the Rosa chinensis 'Old Blush', as well as under four different abiotic treatments, revealed differences in their expression pattern, suggesting that RcGSTs may have different responses to abiotic stresses.
This study provides essential data to support a systematic investigation of the GST gene family within the Rosaceae family and the functional validation of GST genes in roses.
谷胱甘肽S-转移酶（GSTs）在多种生理过程中发挥关键作用，包括植物发育、内源代谢、胁迫耐受性和异源物质解毒。然而，蔷薇科物种中GST基因家族的比较分析及其在玫瑰中的表达模式仍不清楚。
在本研究中，在六种蔷薇科物种中共鉴定出348个GSTs，其中玫瑰（Rosa chinensis）中有78个，草莓（Fragaria vesca）中有53个，桃（Prunus persica）中有69个，李（Prunus mume）中有59个，苹果（Malus domestica）中有43个，梨（Pyrus communis）中有46个。这些GST蛋白被分为九个亚家族。染色体定位、共线性分析和基因复制关系表明，串联重复是蔷薇亚科和桃亚科中GST基因扩增的主要驱动力。基因本体论（GO）和顺式元件分析表明，GST基因可能在多种生物学过程中发挥重要作用，并对各种非生物胁迫作出反应。qRT-PCR分析表明，五个GST基因主要在玫瑰‘老玫瑰’的花组织中表达，以及在四种不同的非生物处理下，其表达模式存在差异，表明RcGSTs可能对非生物胁迫有不同的反应。
本研究提供了重要数据，以支持对蔷薇科内GST基因家族的系统研究以及玫瑰中GST基因的功能验证。
