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RrMYB5 和 RrMYB10 调控的类黄酮生物合成在玫瑰（Rosa rugosa）响应创伤和氧化的反馈环中起着关键作用。

RrMYB5- and RrMYB10-regulated flavonoid biosynthesis plays a pivotal role in feedback loop responding to wounding and oxidation in Rosa rugosa.

机构信息

Key laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China.

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.

出版信息

Plant Biotechnol J. 2019 Nov;17(11):2078-2095. doi: 10.1111/pbi.13123. Epub 2019 Apr 14.

DOI:10.1111/pbi.13123
PMID:30951245
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6790370/
Abstract

Flavonoids play critical roles in plant responses to various stresses. Few studies have been reported on what the mechanism of activating flavonoid biosynthesis in plant responses to wounding and oxidation is. In this study, flavonoid metabolites and many MYB transcript factors from Rosa rugosa were verified to be induced by wounding and oxidation. RrMYB5 and RrMYB10, which belong to PA1- and TT2-type MYB TFs, respectively, showed extremely high induction. Overexpression of RrMYB5 and RrMYB10 resulted in an increased accumulation of proanthocyanidins in R. rugosa and tobacco by promoting the expression of flavonoid structural genes. Transcriptomic analysis of the transgenic plants showed that most genes, involved in wounding and oxidation response and ABA signalling modulation, were up-regulated by the overexpression of RrMYB10, which was very much similar to that observed in RrANR and RrDFR overexpression transgenics. RrMYB5 and RrMYB10 physically interacted and mutually activated each other's expressions. They solely or synergistically activated the different sets of flavonoid pathway genes in a bHLH TF EGL3-independent manner. Eventually, the accumulation of proanthocyanidins enhanced plant tolerance to wounding and oxidative stresses. Therefore, RrMYB5 and RrMYB10 regulated flavonoid synthesis in feedback loop responding to wounding and oxidation in R. rugosa. Our study provides new insights into the regulatory mechanisms of flavonoid biosynthesis by MYB TFs and their essential physiological functions in plant responses to wounding and oxidative stresses.

摘要

类黄酮在植物应对各种胁迫的反应中起着至关重要的作用。关于植物在应对创伤和氧化时激活类黄酮生物合成的机制，目前报道的研究很少。在这项研究中，验证了来自玫瑰的类黄酮代谢物和许多 MYB 转录因子被创伤和氧化诱导。属于 PA1- 和 TT2 型 MYB TF 的 RrMYB5 和 RrMYB10 的诱导作用极强。过表达 RrMYB5 和 RrMYB10 通过促进类黄酮结构基因的表达，导致玫瑰和烟草中原花青素的积累增加。转基因植物的转录组分析表明，大多数与创伤和氧化反应以及 ABA 信号调节相关的基因被 RrMYB10 的过表达上调，这与 RrANR 和 RrDFR 过表达转基因植物中观察到的非常相似。RrMYB5 和 RrMYB10 物理相互作用，并相互激活对方的表达。它们以 bHLH TF EGL3 非依赖性的方式单独或协同激活不同的黄酮类途径基因集。最终，原花青素的积累增强了植物对创伤和氧化胁迫的耐受性。因此，RrMYB5 和 RrMYB10 调节玫瑰中应对创伤和氧化的反馈环中的类黄酮合成。我们的研究为 MYB TF 调节类黄酮生物合成的机制及其在植物应对创伤和氧化应激中的重要生理功能提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01d3/11386786/cff2eb7fc4a2/PBI-17-2078-g004.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01d3/11386786/319511e6134c/PBI-17-2078-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01d3/11386786/0e8abb496470/PBI-17-2078-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01d3/11386786/09759ee75b71/PBI-17-2078-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01d3/11386786/b42edcf1b20e/PBI-17-2078-g005.jpg
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