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从循环肿瘤细胞创建和利用三维胰腺癌模型的方案

Protocol for the creation and utilization of 3D pancreatic cancer models from circulating tumor cells.

作者信息

Tang Jiajia, Zheng Quan, Wang Qi, Zhao Yaru, Ananthanarayanan Preeta, Reina Chiara, Šabanović Berina, Jiang Ke, Yang Ming-Hsin, Meny Clara Csilla, Wang Huimin, Agerbaek Mette Ø, Clausen Thomas Mandel, Gustavsson Tobias, Wen Chenlei, Borghi Felice, Mellano Alfredo, Fenocchio Elisabetta, Gregorc Vanesa, Sapino Anna, Theander Thor G, Fu Da, Aicher Alexandra, Salanti Ali, Shen Baiyong, Heeschen Christopher

机构信息

Center for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; State Key Laboratory of Systems Medicine for Cancer, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.

Center for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; State Key Laboratory of Systems Medicine for Cancer, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.

出版信息

STAR Protoc. 2025 Mar 21;6(1):103635. doi: 10.1016/j.xpro.2025.103635. Epub 2025 Feb 11.

DOI:10.1016/j.xpro.2025.103635
PMID:39946239
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11870243/
Abstract

We introduce a protocol for generating 3D organoids from circulating tumor cells (CTCs), enabling longitudinal functional and molecular analyses in pancreatic cancer patients, including those with unresectable disease, which constitutes the majority of cases. We outline the process for isolating and characterizing CTCs from the blood of pancreatic cancer patients and provide detailed instructions for initiating, passaging, and phenotyping CTC-derived organoids. Additionally, we describe techniques for utilizing these organoids in drug screening with a focus on stemness-related pathways. For complete details on the use and execution of this protocol, please refer to Tang et al..

摘要

我们介绍了一种从循环肿瘤细胞（CTC）生成三维类器官的方案，该方案能够对胰腺癌患者进行纵向功能和分子分析，包括那些患有不可切除疾病的患者，而这类患者占大多数病例。我们概述了从胰腺癌患者血液中分离和鉴定CTC的过程，并提供了启动、传代和鉴定CTC来源类器官的详细说明。此外，我们描述了利用这些类器官进行药物筛选的技术，重点关注与干性相关的信号通路。有关该方案使用和实施的完整详细信息，请参考Tang等人的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/766f/11870243/89405417d24e/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/766f/11870243/6bfb16405b3b/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/766f/11870243/6ecf7cde8689/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/766f/11870243/d8b790a603f5/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/766f/11870243/2596567052aa/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/766f/11870243/cdfca781092e/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/766f/11870243/1bbd18b7aace/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/766f/11870243/89405417d24e/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/766f/11870243/6bfb16405b3b/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/766f/11870243/6ecf7cde8689/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/766f/11870243/d8b790a603f5/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/766f/11870243/2596567052aa/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/766f/11870243/cdfca781092e/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/766f/11870243/1bbd18b7aace/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/766f/11870243/89405417d24e/gr6.jpg

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本文引用的文献

1
CTC-derived pancreatic cancer models serve as research tools and are suitable for precision medicine approaches.CTC 衍生的胰腺癌模型可用作研究工具，并且适合精准医疗方法。
Cell Rep Med. 2024 Sep 17;5(9):101692. doi: 10.1016/j.xcrm.2024.101692. Epub 2024 Aug 19.
2
Cancer organoids 2.0: modelling the complexity of the tumour immune microenvironment.癌症类器官 2.0：模拟肿瘤免疫微环境的复杂性。
Nat Rev Cancer. 2024 Aug;24(8):523-539. doi: 10.1038/s41568-024-00706-6. Epub 2024 Jul 8.
3
The VAR2CSA malaria protein efficiently retrieves circulating tumor cells in an EpCAM-independent manner.
VAR2CSA 疟疾蛋白以 EpCAM 非依赖的方式有效地获取循环肿瘤细胞。
Nat Commun. 2018 Aug 16;9(1):3279. doi: 10.1038/s41467-018-05793-2.
4
Patient-derived organoids model treatment response of metastatic gastrointestinal cancers.患者来源的类器官模型可模拟转移性胃肠道癌症的治疗反应。
Science. 2018 Feb 23;359(6378):920-926. doi: 10.1126/science.aao2774.
5
Targeting Human Cancer by a Glycosaminoglycan Binding Malaria Protein.通过一种糖胺聚糖结合疟原蛋白靶向人类癌症。
Cancer Cell. 2015 Oct 12;28(4):500-514. doi: 10.1016/j.ccell.2015.09.003.
6
Microenvironmental hCAP-18/LL-37 promotes pancreatic ductal adenocarcinoma by activating its cancer stem cell compartment.微环境 hCAP-18/LL-37 通过激活其癌症干细胞隔室促进胰腺导管腺癌。
Gut. 2015 Dec;64(12):1921-35. doi: 10.1136/gutjnl-2014-308935. Epub 2015 Apr 3.