从循环肿瘤细胞创建和利用三维胰腺癌模型的方案
Protocol for the creation and utilization of 3D pancreatic cancer models from circulating tumor cells.
作者信息
Tang Jiajia, Zheng Quan, Wang Qi, Zhao Yaru, Ananthanarayanan Preeta, Reina Chiara, Šabanović Berina, Jiang Ke, Yang Ming-Hsin, Meny Clara Csilla, Wang Huimin, Agerbaek Mette Ø, Clausen Thomas Mandel, Gustavsson Tobias, Wen Chenlei, Borghi Felice, Mellano Alfredo, Fenocchio Elisabetta, Gregorc Vanesa, Sapino Anna, Theander Thor G, Fu Da, Aicher Alexandra, Salanti Ali, Shen Baiyong, Heeschen Christopher
机构信息
Center for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; State Key Laboratory of Systems Medicine for Cancer, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Center for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; State Key Laboratory of Systems Medicine for Cancer, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
出版信息
STAR Protoc. 2025 Mar 21;6(1):103635. doi: 10.1016/j.xpro.2025.103635. Epub 2025 Feb 11.
We introduce a protocol for generating 3D organoids from circulating tumor cells (CTCs), enabling longitudinal functional and molecular analyses in pancreatic cancer patients, including those with unresectable disease, which constitutes the majority of cases. We outline the process for isolating and characterizing CTCs from the blood of pancreatic cancer patients and provide detailed instructions for initiating, passaging, and phenotyping CTC-derived organoids. Additionally, we describe techniques for utilizing these organoids in drug screening with a focus on stemness-related pathways. For complete details on the use and execution of this protocol, please refer to Tang et al..
我们介绍了一种从循环肿瘤细胞（CTC）生成三维类器官的方案，该方案能够对胰腺癌患者进行纵向功能和分子分析，包括那些患有不可切除疾病的患者，而这类患者占大多数病例。我们概述了从胰腺癌患者血液中分离和鉴定CTC的过程，并提供了启动、传代和鉴定CTC来源类器官的详细说明。此外，我们描述了利用这些类器官进行药物筛选的技术，重点关注与干性相关的信号通路。有关该方案使用和实施的完整详细信息，请参考Tang等人的研究。
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