Zhang Qingqing, Zhai Tingting, Guo Yuxin, Weng Yifan, Zhou Nan, Lin Han, Tan Hao, Lu Kangning, Zhou Yiyi
School of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China.
School of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China; College of Food & Biology Engineering, Zhejiang Gongshang University, Hangzhou 310035, PR China.
Food Chem. 2025 May 30;475:143275. doi: 10.1016/j.foodchem.2025.143275. Epub 2025 Feb 7.
Highly selective and sensitive detection of foodborne pathogens is crucial for ensuring food safety. In this work, a photoelectrochemical (PEC) aptasensing platform was developed for the selective and sensitive detection of Vibrio parahaemolyticus (VP), adopting Faraday cage-type construction mode. When VP was present in the sample, the aptamer for VP (Apt) assembled on the Apt/BiS/ITO electrode captured VP, which bound GO@CuO-Apt to the electrode surface. The graphene oxide (GO) in GO@CuO-Apt has a large surface area and good conductivity, on the surface of which a large amount of CuO that can switch the polarity of the BiS photocurrent was loaded, resulting in a highly selective and sensitive detection of VP with a detection limit of 1.0 CFU/mL. By adjusting the Apt, the platform can be used for the detection of other foodborne pathogens, offering broad application potential in foodborne pathogen detection.
