Huang Wei, Liu Fengping, Geng Lianguo, Zhao Shulin, Ye Fanggui
State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), Collaborative Innovation Center for Guangxi Ethnic Medicine, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin, 541004, PR China; Guangxi Key Laboratory for High-value Utilization of Manganese Resources, College of Chemical and Biological Engineering, Guangxi Minzu Normal University, Chongzuo, 532200, PR China.
State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), Collaborative Innovation Center for Guangxi Ethnic Medicine, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin, 541004, PR China; Guangxi Key Laboratory for High-value Utilization of Manganese Resources, College of Chemical and Biological Engineering, Guangxi Minzu Normal University, Chongzuo, 532200, PR China.
Talanta. 2025 May 1;286:127489. doi: 10.1016/j.talanta.2024.127489. Epub 2024 Dec 26.
Sensitive and accurate determination of tumour biomarkers is extremely important for early cancer diagnosis. Herein, a photoelectrochemical biosensor platform was constructed for ultrasensitive tumour biomarker detection by utilizing Au@CuO to switch the photocurrent polarity of CdS/Ni-catecholates metal-organic framework (Ni-CAT) nanorod arrays grown in situ on ITO. The Ni-CAT obtains close contact with ITO and forms a Z-scheme heterojunction with CdS, which improves the photogenerated electron transfer ability. When the target CEA (a model tumour biomarker) was present, CdS/Ni-CAT conjugated with aptamer 1 and Au@CuO conjugated with aptamer 2 to form a sandwich-type complex with the CEA, resulting in the switch of photocurrent polarity of CdS/Ni-CAT from anode to cathode. Owing to the high photogenerated carrier separation efficiency of Au@CuO core-shell heterojunction, the constructed biosensor exhibited ultrasensitive for the detection of CEA with a wide linear range of 0.1 pg·mL-10 ng mL and effectively eliminated false-positive or false-negative signals, which provides a potential alternative solution for the determination of tumour markers in bioanalysis and clinical diagnosis.
灵敏且准确地测定肿瘤生物标志物对于早期癌症诊断极为重要。在此，通过利用Au@CuO来切换在ITO上原位生长的CdS/镍 - 儿茶酚金属有机框架（Ni - CAT）纳米棒阵列的光电流极性，构建了一种用于超灵敏肿瘤生物标志物检测的光电化学生物传感器平台。Ni - CAT与ITO紧密接触并与CdS形成Z型异质结，这提高了光生电子转移能力。当目标癌胚抗原（一种典型的肿瘤生物标志物）存在时，与适配体1共轭的CdS/Ni - CAT和与适配体2共轭的Au@CuO与癌胚抗原形成夹心型复合物，导致CdS/Ni - CAT的光电流极性从阳极切换到阴极。由于Au@CuO核壳异质结具有高光生载流子分离效率，构建的生物传感器对癌胚抗原的检测表现出超灵敏性，线性范围宽达0.1 pg·mL-1至10 ng mL，有效消除了假阳性或假阴性信号，为生物分析和临床诊断中肿瘤标志物的测定提供了一种潜在的替代解决方案。
