Huang Jiamin, Zhao Hong, Chen Xinlian, Lin Tianran, Hou Li, Zhao Shulin
School of Chemistry and Pharmaceutical Sciences, State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Key Laboratory of Environmental Processes and Remediation in Ecologically Fragile Regions, Guangxi Normal University, Guilin 541004 PR China.
School of Chemistry and Pharmaceutical Sciences, State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Key Laboratory of Environmental Processes and Remediation in Ecologically Fragile Regions, Guangxi Normal University, Guilin 541004 PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 May 15;333:125894. doi: 10.1016/j.saa.2025.125894. Epub 2025 Feb 11.
To meet the increasing demands for highly active and stable nanozymes for bioanalysis, Pt nanoparticles (NPs) were successfully supported on the surface of hydrogen-bonded organic frameworks (Pt/HOFs) to obtain a stable, multifunctional and highly active nanozyme for the colorimetric detection of alkaline phosphatase (ALP). Through a redox reaction, Pt precursors are reduced to ultrasmall Pt NPs that are loaded on HOFs. The Pt/HOFs nanozyme showed excellent oxidase-like, peroxidase-like and some catalase-like activity. To avoid the signal instability caused by HO decomposition, the oxidase-like activity of Pt/HOFs was applied for the detection of ALP using 3,3', 5,5 '-tetramethylbenzidine (TMB) as a chromogenic substrate. When the substrate L-ascorbic acid 2-phosphate (AAP) is present, ALP can catalyze AAP to produce strongly reductive ascorbic acid (AA). AA can reduce the oxidation product of TMB. Therefore, a colorimetric sensing strategy for ALP detection was constructed. The linear range of the strategy was from 0.5 to 8 mU mL, and the detection limit was 0.46 mU mL. Finally, the strategy was successfully applied to the detection of ALP in human serum, which provided a reliable strategy for the colorimetric detection of clinical ALP. This study not only presents a simple approach to maintain the high activity of nanoparticle-based nanozymes, but also expands the application of multifunctional HOF-based materials for future applications.
为了满足生物分析对高活性和稳定纳米酶日益增长的需求，成功地将铂纳米颗粒（NPs）负载在氢键有机框架（Pt/HOFs）表面，以获得一种用于比色检测碱性磷酸酶（ALP）的稳定、多功能且高活性的纳米酶。通过氧化还原反应，铂前驱体被还原为负载在HOFs上的超小铂纳米颗粒。Pt/HOFs纳米酶表现出优异的类氧化酶、类过氧化物酶和一些类过氧化氢酶活性。为避免HO分解导致的信号不稳定，利用Pt/HOFs的类氧化酶活性，以3,3',5,5'-四甲基联苯胺（TMB）作为显色底物检测ALP。当存在底物L-抗坏血酸2-磷酸酯（AAP）时，ALP可催化AAP产生强还原性的抗坏血酸（AA）。AA可还原TMB的氧化产物。因此，构建了一种用于检测ALP的比色传感策略。该策略的线性范围为0.5至8 mU/mL，检测限为0.46 mU/mL。最后，该策略成功应用于人血清中ALP的检测，为临床ALP的比色检测提供了可靠策略。本研究不仅提出了一种维持基于纳米颗粒的纳米酶高活性的简单方法，还拓展了基于多功能HOF材料在未来应用中的应用范围。
