Slack J M
Cell. 1985 May;41(1):237-47. doi: 10.1016/0092-8674(85)90077-7.
The regional and temporal specificity of peanut agglutinin binding was determined for early amphibian embryos. With the onset of neurulation, a receptor appears on the epidermis, but remains absent from the neural plate. A second type of receptor, largely masked by sialic acid, appears throughout the extracellular matrix. In the axolotl, the epidermal receptor is epimucin and the matrix receptor is fibronectin plus other components. Both receptors are autonomously expressed, on schedule, by appropriate explants of gastrula tissue. Expression of the epidermal receptor is suppressed after exposure to a neural inducing signal. This shows that the epidermal PNA receptor is a reliable marker of epidermal character and that neural induction affects the program of macromolecular synthesis within hours of the graft.
研究了花生凝集素结合在早期两栖动物胚胎中的区域和时间特异性。随着神经胚形成的开始，表皮上出现一种受体，但神经板上没有。第二种受体主要被唾液酸掩盖，出现在整个细胞外基质中。在蝾螈中，表皮受体是表皮粘蛋白，基质受体是纤连蛋白加其他成分。两种受体都由原肠胚组织的适当外植体按计划自主表达。暴露于神经诱导信号后，表皮受体的表达受到抑制。这表明表皮PNA受体是表皮特征的可靠标志物，并且神经诱导在移植后数小时内影响大分子合成程序。
