Duncan A, Bowie E J, Owen C A, Fass D N
Clin Chem. 1985 Jun;31(6):853-5.
Automated procedures involving a chromogenic substrate sensitive to thrombin-sarcosine-Pro-Arg p-nitroanilide were compared with conventional tests for prothrombin times and activated partial thromboplastin times (APTT) and with specific assays for factors V, VII, VIII, IX, X, XI, and XII. The reproducibility and sensitivity of the chromogenic tests were compared with those of the clotting tests. Further, we have confirmed that the chromogenic test for APTT is sensitive to factor VII deficiency, unlike the clotting test for APTT. This might be an advantage in monitoring orally anticoagulated patients. The ready availability of the automated equipment for performing the chromogenic tests suggests their potential for routine use. However, some discrepant results in certain patients with liver disease and in others with factor VIII inhibitors warrant caution.
将涉及对凝血酶-肌氨酸-脯氨酸-精氨酸对硝基苯胺敏感的显色底物的自动化程序，与凝血酶原时间和活化部分凝血活酶时间（APTT）的传统检测方法，以及因子V、VII、VIII、IX、X、XI和XII的特异性检测方法进行了比较。将显色试验的重现性和敏感性与凝血试验的进行了比较。此外，我们已经证实，与APTT的凝血试验不同，APTT的显色试验对因子VII缺乏敏感。这在监测口服抗凝治疗的患者中可能是一个优势。用于进行显色试验的自动化设备易于获得，表明了其常规使用的潜力。然而，某些肝病患者和其他有因子VIII抑制剂的患者出现的一些不一致结果值得谨慎对待。
