de Metz M, van Wersch J W
De Wever Ziekenhuis, Heerlen, The Netherlands.
Haemostasis. 1987;17(5):254-9. doi: 10.1159/000215752.
A Cobas Bio centrifugal analyzer was used in a clinical laboratory for the performance of chromogenic clotting assays. Three commercially available photometric clotting tests--prothrombin time (PT), activated partial thromboplastin time (aPTT) and fibrinogen--were compared with the traditional clotting assays during 3 months. No great discrepancies were found between the traditional assays and the new photometric assays. The chromogenic PT could replace the traditional thrombotest, PT and Normotest, because it was sensitive and accurate over a broad range of clotting factor activity. Furthermore the chromogenic PT could be used to discriminate between a decreased clotting activity due to vitamin K deficiency or to a decreased protein synthesis by the liver. A decreased protein synthesis was confirmed by measuring a decrease in the serum cholinesterase activity. The chromogenic aPTT could be used for the assay of heparin concentrations in the therapeutic range and turned out to be more sensitive for deficiencies of factor VIII and factor IX than a traditional clotting aPTT. We conclude that the accuracy and practicability of clotting assays are improved by the new assays without diminishing the clinical value of the results.
在一家临床实验室中，使用了一台Cobas Bio离心分析仪进行发色底物法凝血检测。在3个月的时间里，将三种市售的光度法凝血试验——凝血酶原时间（PT）、活化部分凝血活酶时间（aPTT）和纤维蛋白原——与传统凝血试验进行了比较。传统试验和新的光度法试验之间未发现重大差异。发色底物法PT可以替代传统的血栓试验、PT和正常试验，因为它在广泛的凝血因子活性范围内灵敏且准确。此外，发色底物法PT可用于区分因维生素K缺乏导致的凝血活性降低和因肝脏蛋白质合成减少导致的凝血活性降低。通过测量血清胆碱酯酶活性的降低来证实蛋白质合成减少。发色底物法aPTT可用于检测治疗范围内的肝素浓度，并且对于因子VIII和因子IX缺乏症，它比传统的凝血aPTT更敏感。我们得出结论，新的检测方法提高了凝血检测的准确性和实用性，同时不降低结果的临床价值。
