Tripodi A, Mannucci P M
Clin Chem. 1984 Aug;30(8):1392-5.
The clinical usefulness of a chromogenic method for prothrombin time (PT) determination has been assessed in a wide range of clinical conditions, and it is compared with the conventional clotting method for PT. The new method appears to be as sensitive as the clotting PT to deficiencies of clotting factors of the extrinsic and common pathway, except for fibrinogen. Patients with proven liver disease were correctly diagnosed with a prevalence of abnormal results comparable to that by the clotting PT. Results by the two methods correlated highly (r = 0.96) for normal and congenitally deficient plasmas as well as for plasmas from patients on oral anticoagulant treatment (r = 0.95). High reproducibility (between-assay CV less than 3%) and easy adaptation to centrifugal analyzers make it a suitable candidate to replace the conventional method.
已在多种临床情况下评估了一种用于测定凝血酶原时间（PT）的显色法的临床实用性，并将其与传统的PT凝血法进行了比较。除纤维蛋白原外，新方法对外源性和共同途径凝血因子缺乏的敏感性似乎与凝血PT相同。经证实患有肝病的患者被正确诊断，异常结果的患病率与凝血PT相当。两种方法对正常血浆、先天性缺乏血浆以及口服抗凝治疗患者的血浆检测结果高度相关（r = 0.96），口服抗凝治疗患者血浆的两种方法检测结果相关性也很高（r = 0.95）。高重现性（批间变异系数小于3%）以及易于适配离心分析仪使其成为替代传统方法的合适选择。
