Li Chenyang, Wang Ruili, Li Jiana, Zhou Qingyuan, Cui Cui
College of Agronomy and Biotechnology, Southwest University, Chongqing, China.
Front Plant Sci. 2025 Jan 31;16:1546572. doi: 10.3389/fpls.2025.1546572. eCollection 2025.
As soil becomes more acidic, aluminum toxicity has emerged as a key issue impacting seed germination and crop productivity in such environments. Therefore, it is urgent to investigate the mechanism of the influence of aluminum stress on germination. In this study, we focused on one of the major bioenergy crops-rapeseed. Seeds of aluminum-sensitive (S) and aluminum-resistant (R) lines screened from the recombinant inbred lines (RILs) population of rapeseed were treated with 80 µg·ml AlCl (ST, RT). Purified water served as the control (SC, RC). On the 3rd, 5th, and 7th day after treatment, the root tissue was collected for transcriptome sequencing. Utilizing MapMan software, the genes showing differential expression in S and R lines were assigned to the aluminum stress signaling pathway, resulting in the identification of 1036 genes. By weighted gene co-expression network analysis (WGCNA), five co-expressed gene modules associated with aluminum stress were discovered. A total of 332 candidate genes were screened by combining the genes related to aluminum stress signal transduction pathways with the module hub genes. Among them, 26 key genes were located in quantitative trait loci (QTL) with confidence intervals for germination-related traits of rapeseed under aluminum stress, and primarily distributed in 11 QTL regions, such as , and , they were associated with relative root length (RRL), relative root dry weight (RDW), relative germination vigor (RGV) and relative bud length (RBL). The roles included transcription regulation, stress protein production, redox processes, hormone signaling, cell wall alteration, and calcium-based signal transmission. Compared with the R line, the S line exhibited quicker and stronger activation of genes related to aluminum stress signal transduction, suggesting that the S line was more responsive to aluminum stress. This research offers an empirical basis for identifying aluminum-resistant rapeseed varieties and investigating the molecular regulation of aluminum tolerance during germination.
随着土壤酸性增强，铝毒已成为影响此类环境中种子萌发和作物生产力的关键问题。因此，迫切需要研究铝胁迫对种子萌发的影响机制。在本研究中，我们聚焦于主要生物能源作物之一——油菜籽。从油菜籽重组自交系（RILs）群体中筛选出的铝敏感（S）和铝抗性（R）品系的种子，用80 µg·ml AlCl（ST，RT）处理。纯净水作为对照（SC，RC）。处理后第3、5和7天，收集根组织进行转录组测序。利用MapMan软件，将在S和R品系中表现出差异表达的基因分配到铝胁迫信号通路，从而鉴定出1036个基因。通过加权基因共表达网络分析（WGCNA），发现了5个与铝胁迫相关的共表达基因模块。通过将与铝胁迫信号转导途径相关的基因与模块中心基因相结合，共筛选出332个候选基因。其中，26个关键基因位于铝胁迫下油菜籽萌发相关性状的置信区间的数量性状位点（QTL）中，主要分布在11个QTL区域，如 、 和 ，它们与相对根长（RRL）、相对根干重（RDW）、相对萌发活力（RGV）和相对芽长（RBL）相关。其作用包括转录调控、应激蛋白产生、氧化还原过程、激素信号传导、细胞壁改变和钙基信号传递。与R品系相比，S品系对铝胁迫信号转导相关基因的激活更快、更强，表明S品系对铝胁迫更敏感。本研究为鉴定耐铝油菜品种及研究萌发过程中铝耐受性的分子调控提供了实证依据。
