Haskey Natasha, Letef Clara, Sousa James A, Yousuf Munazza, Taylor Lorian M, McKay Derek M, Ma Christopher, Ghosh Subrata, Gibson Deanna L, Raman Maitreyi
Department of Biology, Irving K. Barber Faculty of Science, University of British Columbia-Okanagan, Kelowna, BC, Canada.
Gastrointestinal Research Group, Department of Physiology & Pharmacology, Calvin, Phoebe & Joan Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada.
Therap Adv Gastroenterol. 2025 Feb 16;18:17562848251314827. doi: 10.1177/17562848251314827. eCollection 2025.
Dietary fatty acids (FA) are crucial to the pathophysiology of inflammatory bowel disease (IBD), influencing systemic and gut inflammatory responses. Dietary FA intake influences the fatty acid profiles of vital cell membranes, which might be a source of inflammatory mediators. Despite their significance, research on dietary FA subtypes and their effects on inflammation and oxidative stress in IBD is limited.
We investigated the association between dietary FA intake, the erythrocyte membrane FA composition (EMFA), and inflammation and oxidative stress markers in patients with mild-moderate luminal Crohn's Disease (CD) participating in the CD Therapeutic Dietary Intervention (CD-TDI).
A cross-sectional analysis was performed on 24 participants (13 CD-TDI, 11 habitual diet controls) from a 13-week randomized controlled trial assessing the efficacy of CD-TDI in inducing clinical and biomarker remission in CD.
EMFA was analyzed using direct-injection gas chromatography, and dietary FA intake was assessed using the ASA 24-h Dietary Assessment Tool.
The CD-TDI group showed a significant increase in dietary n-3 polyunsaturated fatty acids (PUFA) at Week 13 ( = 0.04) compared to no changes in the control group. Participants on the CD-TDI also demonstrated a significant reduction in total fat, saturated fat, and arachidonic acid (AA) ( < 0.01). EMFA analysis revealed lower percentages of AA ( = 0.03) in the CD-TDI group. Positive correlations were observed between C-reactive protein, fecal calprotectin, and dietary stearic acid ( < 0.05). Inverse correlations were found between malondialdehyde (MDA) and the Mediterranean Diet Score ( = -0.67) as well as MDA and the intake of whole fruit, legumes, and nuts/seeds ( > -0.50).
The CD-TDI significantly increased dietary n-3 PUFA intake, reduced pro-inflammatory n-6 PUFA (AA), and improved markers of oxidative stress, supporting its potential in CD management. The cell membrane fatty acid profile might be a therapeutic target in CD.
NCT04596566.
膳食脂肪酸（FA）对炎症性肠病（IBD）的病理生理学至关重要，影响全身和肠道炎症反应。膳食FA摄入量会影响重要细胞膜的脂肪酸谱，这可能是炎症介质的一个来源。尽管其具有重要意义，但关于膳食FA亚型及其对IBD炎症和氧化应激影响的研究有限。
我们调查了参与克罗恩病治疗性饮食干预（CD-TDI）的轻度至中度肠腔型克罗恩病（CD）患者的膳食FA摄入量、红细胞膜FA组成（EMFA）与炎症和氧化应激标志物之间的关联。
对一项为期13周的随机对照试验中的24名参与者（13名CD-TDI参与者，11名习惯饮食对照组）进行横断面分析，该试验评估了CD-TDI在诱导CD患者临床和生物标志物缓解方面的疗效。
使用直接进样气相色谱法分析EMFA，并使用ASA 24小时膳食评估工具评估膳食FA摄入量。
与对照组无变化相比，CD-TDI组在第13周时膳食n-3多不饱和脂肪酸（PUFA）显著增加（P = 0.04）。接受CD-TDI的参与者的总脂肪、饱和脂肪和花生四烯酸（AA）也显著减少（P < 0.01）。EMFA分析显示CD-TDI组中AA的百分比更低（P = 0.03）。观察到C反应蛋白、粪便钙卫蛋白与膳食硬脂酸之间存在正相关（P < 0.05）。发现丙二醛（MDA）与地中海饮食评分之间存在负相关（P = -0.67），以及MDA与全水果、豆类和坚果/种子的摄入量之间存在负相关（P > -0.50）。
CD-TDI显著增加了膳食n-3 PUFA摄入量，减少了促炎n-6 PUFA（AA），并改善了氧化应激标志物，支持其在CD管理中的潜力。细胞膜脂肪酸谱可能是CD的一个治疗靶点。
NCT04596566。
