Signore Anthony V, Joseph Tomy, Ranadheera Charlene, Erdelyan Cassidy N G, Alkie Tamiru N, Raj Sugandha, Pama Lemarie, Ayilara Ifeoluwa, Hisanaga Tamiko, Lung Oliver, Bastien Nathalie, Berhane Yohannes
National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, Canada.
Animal Health Centre, BC Ministry of Agriculture and Food, Abbotsford, Canada.
Emerg Microbes Infect. 2025 Dec;14(1):2469643. doi: 10.1080/22221751.2025.2469643. Epub 2025 Mar 11.
We report the detection of a clade 2.3.4.4b A(H5N1) reassortant virus with a neuraminidase surface protein derived from a North American lineage low-pathogenic avian influenza virus. This virus caused a widespread and ongoing outbreak across 45 poultry farms in British Columbia, Canada. Isolates from 8 farms reveal a mutation in the neuraminidase protein (H275Y) that is exceptionally rare among clade 2.3.4.4b viruses (present in 0.045% of publicly available clade 2.3.4.4b isolates). NA-H275Y is a well-known marker of resistance to the neuraminidase inhibitor oseltamivir. We demonstrate that this substitution maintains its resistance phenotype on the genetic background of H5N1 clade 2.3.4.4b viruses.
我们报告检测到一种2.3.4.4b分支A(H5N1)重配病毒，其神经氨酸酶表面蛋白源自北美谱系低致病性禽流感病毒。该病毒在加拿大不列颠哥伦比亚省的45个家禽养殖场引发了一场广泛且持续的疫情。来自8个养殖场的分离株显示神经氨酸酶蛋白发生了突变（H275Y），这在2.3.4.4b分支病毒中极为罕见（在公开可用的2.3.4.4b分支分离株中占0.045%）。NA-H275Y是对神经氨酸酶抑制剂奥司他韦耐药的一个众所周知的标志物。我们证明这种替换在H5N1 2.3.4.4b分支病毒的遗传背景上保持了其耐药表型。
