Akgun-Unal Nı̇lufer, Ustun Aylı̇n, Bayirli Sevban, Unal Omer, Mogulkoc Rası̇m, Baltaci Abdulkerim Kasım
Medical Faculty Department of Biophysics, Ondokuz Mayıs University, Samsun, Turkey.
Selcuk University Vocational School of Health Services, Konya, Turkey.
Biol Trace Elem Res. 2025 Feb 18. doi: 10.1007/s12011-025-04550-z.
In this study, we aimed to investigate the effects of cumulative doses of Zn (by exposing samples to 1 µM, 10 µM, and 100 µM ZnCl) on myocardial papillary muscle contractions isolated from rat hearts in vitro and the roles of the zinc finger protein ZEB1 in this effect. In these preparations, 100 µM ZnCl application in different protocols caused a decrease in contraction force and an increase in contraction time in both frequency-dependent parameters and pre-expected stimuli when compared to the control group. Our study data show that Ca homeostasis is closely related to increasing Zn doses (especially at 100 µM ZnCl dose). Secondly, the levels of ZEB1, a zinc finger protein, were also significantly lower in the 100 µM ZnCl group compared to the other groups, which seems to be related to the increase in Ca that triggers ROS production at high doses of Zn. The data of our study, which we conducted to understand the Zn concentrations in the heart and to reveal new mechanisms that play a role in the regulation of Ca dynamics in heart tissue and is the first research in the literature on this subject, show that in vitro zinc application may have a dose-dependent effect on myocardial papillary muscle contractions.
在本研究中，我们旨在研究累积剂量的锌（通过将样本暴露于1µM、10µM和100µM的氯化锌中）对体外分离的大鼠心脏心肌乳头肌收缩的影响，以及锌指蛋白ZEB1在此效应中的作用。在这些制剂中，与对照组相比，在不同方案中应用100µM氯化锌导致收缩力降低，并且在频率依赖性参数和预预期刺激下的收缩时间增加。我们的研究数据表明，钙稳态与锌剂量增加密切相关（尤其是在100µM氯化锌剂量时）。其次，与其他组相比，100µM氯化锌组中锌指蛋白ZEB1的水平也显著降低，这似乎与高剂量锌引发ROS产生时钙的增加有关。我们进行这项研究是为了了解心脏中的锌浓度，并揭示在心脏组织中钙动力学调节中起作用的新机制，这是该主题文献中的首次研究，我们的研究数据表明，体外应用锌可能对心肌乳头肌收缩有剂量依赖性影响。
