Abdelhameed Reda E, Hegazy Hegazy S, Abdalla Hanan, Adarosy Marwa H
Botany and Microbiology Department, Faculty of Science, Zagazig, University, Zagazig, 44519, Egypt.
BMC Plant Biol. 2025 Feb 18;25(1):221. doi: 10.1186/s12870-025-06194-6.
Salinity is among the major abiotic stresses faced by different countries; limiting plant growth, development and yield. This research work was carried out to evaluate the influence of green prepared titanium dioxide nanoparticles (TiO NPs) on the growth, metabolic constituents and ultrastructural alterations of soybean (Glycine max L.) plants exposed to salt stress. TiO NPs were green synthesized using an aqueous solution of Aloe vera leaf extract and the obtained NPs were identified using several techniques. An in vivo pot experiment was carried out to evaluate the role of foliar sprayed TiO NPs (30 ppm) on soybean plants irrigated by six NaCl concentrations (0, 25, 50, 100, 150 and 200 mM). After 15 and 30 days from salt application, growth parameters, photosynthetic pigments, total soluble protein, enzymatic antioxidants and ultrastructural changes were tested for potential tolerance of soybean plants growing under salt stress. Results revealed that increasing salt concentrations induced a significant decrease in shoot length, fresh and dry weights as well as the photosynthetic pigments, these decreases were due to increasing electrolyte leakage of soybean plants. However, application of TiO NPs showed improvements in the vegetative growth by increasing its pigments and protein contents. There was a marked increase in the contents of enzymatic antioxidants in salt stressed soybean plants and further accumulation of their contents with TiO NPs application. Salt stressed soybean plants showed structural and ultrastructural deformation which was lessened by TiO NPs application. Finally, our research demonstrates the role of TiO NPs in alleviating salt stress in soybean plants via restoring the antioxidants and cell ultrastructure, highlighting their potential role as a sustainable and eco-friendly strategy.
盐度是不同国家面临的主要非生物胁迫之一，限制了植物的生长、发育和产量。本研究旨在评估绿色制备的二氧化钛纳米颗粒（TiO NPs）对遭受盐胁迫的大豆（Glycine max L.）植株的生长、代谢成分和超微结构变化的影响。使用芦荟叶提取物水溶液绿色合成TiO NPs，并采用多种技术对所得纳米颗粒进行鉴定。进行了一项体内盆栽试验，以评估叶面喷施TiO NPs（30 ppm）对用六种NaCl浓度（0、25、50、100、150和200 mM）灌溉的大豆植株的作用。在施加盐分15天和30天后，测试了生长参数、光合色素、总可溶性蛋白、酶促抗氧化剂和超微结构变化，以了解盐胁迫下大豆植株的潜在耐受性。结果表明，盐浓度的增加导致茎长、鲜重和干重以及光合色素显著下降，这些下降是由于大豆植株电解质渗漏增加所致。然而，TiO NPs的施用通过增加色素和蛋白质含量改善了营养生长。盐胁迫大豆植株中酶促抗氧化剂的含量显著增加，施用TiO NPs后其含量进一步积累。盐胁迫大豆植株表现出结构和超微结构变形，而TiO NPs的施用减轻了这种变形。最后，我们的研究证明了TiO NPs通过恢复抗氧化剂和细胞超微结构在缓解大豆植株盐胁迫中的作用，突出了它们作为一种可持续和生态友好策略的潜在作用。
