Mirzaei Alireza, Fooladi Jamshid, Fazeli-Nasab Bahman, Ghorbanpour Mansour
Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.
Department of Agronomy and Plant Breeding, Agriculture Institute, Research Institute of Zabol, Zabol, Iran.
Funct Integr Genomics. 2025 Jul 1;25(1):141. doi: 10.1007/s10142-025-01647-5.
Salt stress is a significant factor limiting plant growth and can severely reduce crop yields. In addition to causing physiological damage, salt stress disrupts the plant's internal balance and hinders nutrient absorption. However, plants have developed various responses to combat this stress. This study focuses on one critical gene associated with the jasmonic acid pathway, known as BnMYC2, which plays an important role in enhancing salt resistance. The BnMYC2 gene activates downstream genes by binding to specific cis-acting element regions, thereby contributing to the plant's ability to withstand salt stress. We investigated the expression of the BnMYC2 gene in the roots, stems, and leaves of the Licord cultivar under salt stress. The experiment was designed completely randomly, with three replications. Our results indicate that BnMYC2 gene expression is higher in the roots compared to the stems and leaves. Notably, the expression level of the BnMYC2 gene peaked in the roots 12 h after the application of salt stress before subsequently decreasing. Bioinformatics analysis revealed that the BnMYC2 gene shares phylogenetic similarities with 16 genes in Arabidopsis thaliana and 8 genes in Glycine max. Additionally, the expression profile of the MYC2 gene in A. thaliana is 72.22% similar to that in the G. max genome. Overall, this research not only highlights the role of the MYC2 gene in salt tolerance but also provides valuable insights into the evolutionary aspects of the BnMYC2 gene, which can be beneficial for future studies related to genome analysis.
盐胁迫是限制植物生长的一个重要因素，会严重降低作物产量。除了造成生理损伤外，盐胁迫还会破坏植物的内部平衡并阻碍养分吸收。然而，植物已经进化出各种应对这种胁迫的反应。本研究聚焦于与茉莉酸途径相关的一个关键基因，即BnMYC2，它在增强耐盐性方面发挥着重要作用。BnMYC2基因通过与特定的顺式作用元件区域结合来激活下游基因，从而有助于植物抵御盐胁迫的能力。我们研究了盐胁迫下Licord品种根、茎和叶中BnMYC2基因的表达情况。实验设计完全随机，有三个重复。我们的结果表明，与茎和叶相比，BnMYC2基因在根中的表达更高。值得注意的是，在施加盐胁迫12小时后，BnMYC2基因在根中的表达水平达到峰值，随后下降。生物信息学分析表明，BnMYC2基因与拟南芥中的16个基因和大豆中的8个基因具有系统发育相似性。此外，拟南芥中MYC2基因的表达谱与大豆基因组中的表达谱相似度为72.22%。总体而言，这项研究不仅突出了MYC2基因在耐盐性中的作用，还为BnMYC2基因的进化方面提供了有价值的见解，这对未来与基因组分析相关的研究可能是有益的。
