Zhou Qilu, Ali Sikandar, Shi Xueyan, Cao Guangtian, Feng Jie, Yang Caimei, Zhang Ruiqiang
College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang Agricultural and Forestry University, Hangzhou 311300, Zhejiang, China.
Zhejiang Vegamax Biotechnology Co., Ltd., Anji 313300, Zhejiang, China.
Anim Nutr. 2024 Nov 20;20:158-170. doi: 10.1016/j.aninu.2024.11.001. eCollection 2025 Mar.
This research explored the protective impact of bamboo leaf flavonoids (BLF) in diquat (DQ) stressed broilers; providing insight of antioxidant, immune response and intestinal barrier function. This experiment consisted of two parts. In the first, 240 chicks were allotted to 2 groups with 8 replicates and 15 chicks per replicate. Treatments consisted of a basic feed (control group, CON) and the basic feed plus 1000 mg/kg BLF (BLF group, BLF) for 28 d, respectively. Then, following the conclusion of the first part, 16 healthy broilers were selected from the CON group and the BLF group. They formed the second part of the experiment, and were allotted to 4 treatments with 8 broilers each: CON-no stress (CON-NS) group, CON-DQ group, BLF-NS group and the BLF-DQ group. Broilers were separately injected intraperitoneally with DQ solution at 40 mg/kg body weight or the same dose of phosphate buffer saline. The results revealed adding BLF to diet reduced the ratio of feed to weight gain of broilers compared to the basic feed group ( = 0.021). In comparison to the CON-NS group, BLF improved the levels of serum and jejunal mucosa total antioxidant capacity, immunoglobulin M, serum catalase, immunoglobulin A, interleukin 10, jejunal mucosa interleukin 4, cecal butyric acid, valeric acid, isobutyric acid, isovaleric acid, upregulated zonula occludens-1 (), occludin () and claudin-1 () expressions, and reduced the levels of jejunal mucosa malondialdehyde (MDA), interleukin 1β, interleukin 6 and serum diamine oxidase ( < 0.05). Diquat stress elevated the contents of serum MDA, D-lactate, jejunal mucosa tumor necrosis factor α, reactive oxygen species and relative abundance, downregulated , and expressions, and reduced Sobs, Chao and Ace indices ( < 0.05). Compared with CON-NS group, the concentration of isovaleric acid in the BLF-DQ group was higher ( < 0.05). In conclusion, by establishing a DQ stress injury model, it was elucidated that BLF may enhance antioxidant capacity, strengthen immunity, regulate volatile fatty acid contents, improve intestinal morphology, microbiota and other intestinal barrier functions, so as to mitigate the injury induced by oxidative stress in broilers.
本研究探讨了竹叶黄酮（BLF）对百草枯（DQ）应激肉鸡的保护作用；深入了解其抗氧化、免疫反应和肠道屏障功能。本实验分为两部分。第一部分，将240只雏鸡分为2组，每组8个重复，每个重复15只雏鸡。处理分别为基础饲料（对照组，CON）和基础饲料加1000 mg/kg BLF（BLF组，BLF），持续28天。然后，在第一部分结束后，从CON组和BLF组中选出16只健康肉鸡。它们构成了实验的第二部分，并被分为4个处理组，每组8只肉鸡：CON-无应激（CON-NS）组、CON-DQ组、BLF-NS组和BLF-DQ组。分别给肉鸡腹腔注射40 mg/kg体重的DQ溶液或相同剂量的磷酸盐缓冲盐水。结果显示，与基础饲料组相比，在日粮中添加BLF降低了肉鸡的料重比（P = 0.021）。与CON-NS组相比，BLF提高了血清和空肠黏膜总抗氧化能力、免疫球蛋白M、血清过氧化氢酶、免疫球蛋白A、白细胞介素10、空肠黏膜白细胞介素4、盲肠丁酸、戊酸、异丁酸、异戊酸水平，上调了紧密连接蛋白-1（ZO-1）、闭合蛋白（Occludin）和Claudin-1（Claudin-1）的表达，并降低了空肠黏膜丙二醛（MDA）、白细胞介素1β、白细胞介素6和血清二胺氧化酶水平（P < 0.05）。百草枯应激提高了血清MDA、D-乳酸、空肠黏膜肿瘤坏死因子α、活性氧和相对丰度，下调了ZO-1、Occludin和Claudin-1的表达，并降低了Sobs、Chao和Ace指数（P < 0.05）。与CON-NS组相比，BLF-DQ组的异戊酸浓度更高（P < 0.05）。总之，通过建立百草枯应激损伤模型，阐明了BLF可能增强抗氧化能力、增强免疫力、调节挥发性脂肪酸含量、改善肠道形态、微生物群和其他肠道屏障功能，从而减轻肉鸡氧化应激诱导的损伤。
