Gao Mingkun, Liao Chaoyong, Fu Jianyang, Ning Zhonghua, Lv Zengpeng, Guo Yuming
State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
J Anim Sci Biotechnol. 2024 Feb 18;15(1):25. doi: 10.1186/s40104-023-00974-6.
Baicalin and probiotic cocktails are promising feed additives with broad application prospects. While probiotic cocktails are known to enhance intestinal health, the potential synergistic impact of combining baicalin with probiotic cocktails on the gut health of broiler chickens remains largely unexplored. Therefore, this study aims to investigate the influence of the combined administration of baicalin and probiotic cocktails on the composition of ileal and cecal microbiota in broiler chickens to elucidate the underlying mechanisms responsible for the health-promoting effects.
A total of 320 1-day-old male Arbor Acres broilers were divided into 4 groups, each with 8 replicates of 10 chicks per replicate. Over a period of 42 d, the birds were fed a basal diet or the same diet supplemented with 37.5 g/t baicalin (BC), 1,000 g/t probiotic cocktails (PC), or a combination of both BC (37.5 g/t) and PC (1,000 g/t). The results demonstrated that BC + PC exhibited positive synergistic effects, enhancing intestinal morphology, immune function, and barrier function. This was evidenced by increased VH/CD ratio, sIgA levels, and upregulated expression of occludin and claudin-1 (P < 0.05). 16S rRNA analysis indicated that PC potentiated the effects of BC, particularly in the ileum, where BC + PC significantly increased the α-diversity of the ileal microbiota, altered its β-diversity, and increased the relative abundance of Flavonifractor (P < 0.05), a flavonoid-metabolizing bacterium. Furthermore, Flavonifractor positively correlated with chicken ileum crypt depth (P < 0.05). While BC + PC had a limited effect on cecal microbiota structure, the PC group had a very similar microbial composition to BC + PC, suggesting that the effect of PC at the distal end of the gut overshadowed those of BC.
We demonstrated the synergistic enhancement of gut health regulation in broiler chickens by combining baicalin and probiotic cocktails. Probiotic cocktails enhanced the effects of baicalin and accelerated its metabolism in the ileum, thereby influencing the ileal microbiota structure. This study elucidates the interaction mechanism between probiotic cocktails and plant extract additives within the host microbiota. These findings provide compelling evidence for the future development of feed additive combinations.
黄芩苷和益生菌鸡尾酒是具有广阔应用前景的饲料添加剂。虽然已知益生菌鸡尾酒可促进肠道健康，但黄芩苷与益生菌鸡尾酒联合使用对肉鸡肠道健康的潜在协同影响在很大程度上仍未得到探索。因此，本研究旨在探究黄芩苷与益生菌鸡尾酒联合给药对肉鸡回肠和盲肠微生物群组成的影响，以阐明其促进健康作用的潜在机制。
总共320只1日龄雄性艾维茵肉鸡被分为4组，每组8个重复，每个重复10只鸡。在42天的时间里，给鸡饲喂基础日粮或添加37.5 g/t黄芩苷（BC）、1000 g/t益生菌鸡尾酒（PC）或两者组合（BC 37.5 g/t + PC 1000 g/t）的相同日粮。结果表明，BC + PC表现出正协同效应，增强了肠道形态、免疫功能和屏障功能。这通过增加VH/CD比值、sIgA水平以及上调闭合蛋白和Claudin-1的表达得到证实（P < 0.05）。16S rRNA分析表明，PC增强了BC的作用，特别是在回肠中，BC + PC显著增加了回肠微生物群的α多样性，改变了其β多样性，并增加了黄酮分解菌（P < 0.05）的相对丰度。此外，黄酮分解菌与鸡回肠隐窝深度呈正相关（P < 0.05）。虽然BC + PC对盲肠微生物群结构的影响有限，但PC组的微生物组成与BC + PC非常相似，这表明PC在肠道远端的作用超过了BC。
我们证明了黄芩苷与益生菌鸡尾酒联合使用可协同增强肉鸡肠道健康调节。益生菌鸡尾酒增强了黄芩苷的作用，并加速了其在回肠中的代谢，从而影响回肠微生物群结构。本研究阐明了益生菌鸡尾酒与植物提取物添加剂在宿主微生物群中的相互作用机制。这些发现为未来饲料添加剂组合的开发提供了有力证据。
