Gallina D L, Pelletier D, Doherty P, Koevary S B, Williams R M, Like A A, Chick W L, Rossini A A
Diabetologia. 1985 Mar;28(3):143-7. doi: 10.1007/BF00273861.
Autologous transfusions of 111indium-labelled peripheral blood lymphocytes reportedly image the pancreas of patients with Type 1 (insulin-dependent) diabetes at the time of onset. We attempted to apply this technique to the spontaneously diabetic BB/W rat. First, acutely diabetic BB/W rats, diabetes-prone BB/W rats, diabetes-resistant W-line BB/W rats, and Wistar Furth rats were given autologous transfusions of labelled peripheral blood lymphocytes. Radioactivity recovered from the pancreas was similar in all groups. No correlation was found between the intensity of imaging and the presence or intensity of insulitis. To decrease non-specific intravascular radioactivity, acutely diabetic, diabetes-prone, and W-line rats were perfused 48 h after autologous transfusion of labelled lymphocytes. Again, the intensity of recovered activity was similar in all groups, using both macroautoradiography and numerical counting techniques. A second set of experiments studied diabetes and insulitis induced by passive transfer of concanavalin A-treated splenic lymphocytes from acutely diabetic donors. Activated lymphocytes were labelled with 111Indium and given to groups of diabetes-prone and diabetes-resistant rats. There were no differences in pancreatic localization 72-96 h after injection. Groups of diabetes-prone and diabetes-resistant rats were also given concanavalin A-activated lymphocytes and then challenged 2-10 days later with autologous transfusions of labelled peripheral blood lymphocytes. Again, no differences in organ labelling or imaging were detected. We conclude that the autologous transfusions of 111indium-labelled lymphocytes do not label or image the pancreas of the BB/W rat.
据报道，111铟标记的外周血淋巴细胞自体输血可在发病时对1型（胰岛素依赖型）糖尿病患者的胰腺进行成像。我们试图将该技术应用于自发性糖尿病BB/W大鼠。首先，给急性糖尿病BB/W大鼠、糖尿病易感BB/W大鼠、糖尿病抵抗W系BB/W大鼠和Wistar Furth大鼠进行标记外周血淋巴细胞的自体输血。所有组从胰腺回收的放射性相似。未发现成像强度与胰岛炎的存在或强度之间存在相关性。为了降低非特异性血管内放射性，在标记淋巴细胞自体输血48小时后，对急性糖尿病、糖尿病易感和W系大鼠进行灌注。同样，使用宏观放射自显影和数值计数技术，所有组回收活性的强度相似。第二组实验研究了由急性糖尿病供体的伴刀豆球蛋白A处理的脾淋巴细胞被动转移诱导的糖尿病和胰岛炎。活化的淋巴细胞用111铟标记，并给予糖尿病易感和糖尿病抵抗大鼠组。注射后72 - 96小时胰腺定位无差异。糖尿病易感和糖尿病抵抗大鼠组也给予伴刀豆球蛋白A活化的淋巴细胞，然后在2 - 10天后用标记外周血淋巴细胞的自体输血进行攻击。同样，未检测到器官标记或成像的差异。我们得出结论，111铟标记淋巴细胞的自体输血不会标记或成像BB/W大鼠的胰腺。
