Somuncu Ahmet Mehmet, Parlak Somuncu Busra, Ozbay Ahmet Duhan, Cicek Ibrahim, Suleyman Bahadir, Mammadov Renad, Bulut Seval, Bal Tastan Tugba, Coban Taha Abdulkadir, Suleyman Halis, Aydin Aliyev
Trabzon Kanuni Education and Research Hospital, Health Sciences University, Trabzon 61250, Turkiey.
Provincial Health Directorate, Ministry of Health of the Republic of Turkey, Trabzon 61040, Turkiey.
Int J Ophthalmol. 2025 Feb 18;18(2):222-228. doi: 10.18240/ijo.2025.02.04. eCollection 2025.
To investigate the effects of adenosine triphosphate (ATP) and melatonin, which have antioxidant and anti-inflammatory activities, on potential 5-fluorouracil (5-FU)-induced optic nerve damage in rats.
Twenty-four rats were categorized into four groups of six rats: healthy (HG), 5-FU (FUG), ATP+5-FU (AFU), and melatonin+5-FU (MFU). ATP (4 mg/kg) and melatonin (10 mg/kg) were administered intraperitoneally and orally, respectively. One hour after ATP and melatonin administration, rats in the AFU, MFU, and FUG were intraperitoneally injected with 5-FU (100 mg/kg). ATP and melatonin were administered once daily for 10d. 5-FU was administered at a single dose on days 1, 3, and 5 of the experiment. After 10d, the rats were euthanized and optic nerve tissues were extracted. Optic nerve tissues were biochemically and histopathologically examined.
ATP and melatonin treatments inhibited the increase in malondialdehyde (MDA) and interleukin-6 (IL-6) levels, which were elevated in the FUG. The treatments also prevented the decrease in total glutathione (tGSH) levels and the superoxide dismutase (SOD) and catalase (CAT) activities (<0.001). This inhibition was higher in the ATP group than in the melatonin group (<0.001). ATP prevented histopathological damage better than melatonin (<0.05).
ATP and melatonin have the potential to be used in alleviating 5-FU-induced optic nerve damage. In addition, ATP treatment shows better protective effects than melatonin.
研究具有抗氧化和抗炎活性的三磷酸腺苷（ATP）和褪黑素对5-氟尿嘧啶（5-FU）诱导的大鼠视神经损伤的潜在影响。
将24只大鼠分为四组，每组6只：健康组（HG）、5-FU组（FUG）、ATP + 5-FU组（AFU）和褪黑素 + 5-FU组（MFU）。ATP（4mg/kg）和褪黑素（10mg/kg）分别通过腹腔注射和口服给药。在给予ATP和褪黑素1小时后，AFU组、MFU组和FUG组的大鼠腹腔注射5-FU（100mg/kg）。ATP和褪黑素每天给药1次，持续10天。在实验的第1、3和5天给予5-FU单次剂量。10天后，对大鼠实施安乐死并提取视神经组织。对视神经组织进行生化和组织病理学检查。
ATP和褪黑素治疗抑制了FUG组中升高的丙二醛（MDA）和白细胞介素-6（IL-6）水平的增加。这些治疗还防止了总谷胱甘肽（tGSH）水平以及超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性的降低（<0.001）。ATP组的这种抑制作用高于褪黑素组（<0.001）。ATP在预防组织病理学损伤方面比褪黑素表现更好（<0.05）。
ATP和褪黑素具有用于减轻5-FU诱导的视神经损伤的潜力。此外，ATP治疗显示出比褪黑素更好的保护作用。
