Abbasluo Mahbobe, Bakhshi Ardakani Mahya, Jafari Negar, Pazoki Mahboubeh
Department of Biology, Islamic Azad University, Ashkezar Branch, Ashkezar, Iran.
Iran University of Medical Sciences , Tehran, Iran.
Med J Islam Repub Iran. 2024 Nov 5;38:128. doi: 10.47176/mjiri.38.128. eCollection 2024.
Coronary artery disease (CAD) is one of the heart diseases that causes the death of many patients in the world. Many genes and molecular pathways are involved in the regulation of inflammation. However, some genes have a regulatory role and control immune responses. In recent studies, few studies have been done regarding the role of TLRs and PPARs in CAD. Hence, the present study aimed to determine and compare the mRNA expression of and genes and genes of the innate immune system messenger pathway, including TLR2and TLR4, in CAD patients in comparison to normal individuals.
This study (case-control) was conducted on 12 patients with coronary arteries and 10 healthy individuals as healthy controls. RNA extraction was performed, cDNA was produced, and then the mRNA expression levels of , , -α, and -γ genes were examined using Syber green Real-Time PCR. The t-test sample and the related non-parametric tests were used to investigate the relationship between the quantitative variables. The significance level in all tests was considered as less than 0.05.
The results of data analysis showed that the expression level of and genes was significantly increased in the patient group compared to the controls (=0.001). However, although -α and -γ genes were up-regulated in patients' samples, the comparison of gene expression levels did not significantly differ between the case and control groups.
we found meaningful results to the significant role of 2 and TLR4 in the pathogenesis of CAD and emphasize the hypothesis that TLR2 and TLR4 can be considered therapeutic options.
冠状动脉疾病（CAD）是导致全球众多患者死亡的心脏病之一。许多基因和分子途径参与炎症调节。然而，一些基因具有调节作用并控制免疫反应。在最近的研究中，关于Toll样受体（TLRs）和过氧化物酶体增殖物激活受体（PPARs）在CAD中的作用的研究较少。因此，本研究旨在确定并比较CAD患者与正常个体相比，先天免疫系统信使途径的TLR2和TLR4等基因以及PPAR-α和PPAR-γ基因的mRNA表达。
本病例对照研究对12例冠状动脉疾病患者和10名健康个体作为健康对照进行。进行RNA提取，产生cDNA，然后使用Syber green实时荧光定量PCR检测PPAR-α、PPAR-γ、TLR2和TLR4基因的mRNA表达水平。使用t检验样本和相关的非参数检验来研究定量变量之间的关系。所有检验的显著性水平均设定为小于0.05。
数据分析结果显示，与对照组相比，患者组中TLR2和TLR4基因的表达水平显著升高（P = 0.001）。然而，尽管患者样本中PPAR-α和PPAR-γ基因上调，但病例组和对照组之间基因表达水平的比较没有显著差异。
我们发现TLR2和TLR4在CAD发病机制中具有重要作用，这一结果具有重要意义，并强调了TLR2和TLR4可被视为治疗选择的假设。
