Verheijen R H, Feitz W F, Beck J L, Debruyne F M, Vooys G P, Kenemans P, Herman C J
Int J Cancer. 1985 May 15;35(5):653-7. doi: 10.1002/ijc.2910350514.
Thirty-six ovarian and renal-cell tumours were analyzed by flow cytometry (FCM) for DNA content and in parallel were assayed for colony formation in a human tumour cloning system (HTCS). While 15/19 (79%) tumours with an abnormal (aneuploid) DNA stemline formed colonies in the HTCS, only 2/17 (12%) of diploid tumours formed colonies. All samples contained tumour cells as assessed by routine cytological examination. The capacity to form colonies in the HTCS was not correlated in these tumours with grade or stage of disease or tumour type. The level of aneuploidy expressed as the FCM DNA index did not correlate with the cloning efficiency in HTCS. These findings suggest that tumour growth in the HTCS reflects a biologically important potential, related in at least some tumours to an abnormal DNA stemline.
采用流式细胞术（FCM）分析了36例卵巢和肾细胞肿瘤的DNA含量，并同时在人肿瘤克隆系统（HTCS）中检测其集落形成能力。在19例具有异常（非整倍体）DNA干系的肿瘤中，有15例（79%）在HTCS中形成了集落，而二倍体肿瘤中只有2例（12%）形成了集落。通过常规细胞学检查评估，所有样本均含有肿瘤细胞。在这些肿瘤中，HTCS中的集落形成能力与疾病的分级、分期或肿瘤类型无关。以FCM DNA指数表示的非整倍体水平与HTCS中的克隆效率无关。这些发现表明，HTCS中的肿瘤生长反映了一种生物学上重要的潜能，至少在某些肿瘤中与异常DNA干系有关。
