Erba E, Ubezio P, Pepe S, Vaghi M, Marsoni S, Torri W, Mangioni C, Landoni F, D'Incalci M
Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy.
Br J Cancer. 1989 Jul;60(1):45-50. doi: 10.1038/bjc.1989.217.
A total of 155 samples from 101 patients with ovarian cancer were investigated using flow cytometry to evaluate the DNA index and the percentage of cells in the various cell cycle phases. Thirty-four samples were DNA diploid tumours, while the other 121 were DNA aneuploid tumours. The DNA index was very stable in different sites and over time in the same patient. Tumour stage and ploidy were significantly associated: stages III and IV tumour stage were more likely to be DNA aneuploid. Patients with residual tumour size at first surgery greater than 2 cm had a significantly larger number of DNA aneuploid than DNA diploid tumours. The DNA index was also related to the degree of differentiation of the tumours. The percentage of cells in the S phase of the cell cycle was significantly higher in DNA aneuploid and in poorly differentiated tumours than DNA diploid and well differentiated tumours. Multivariate analysis using the Cox model showed that the DNA index and the percentage of cells in S phase were not independent prognostic variables in this study. Prospectively collected data should be accumulated before assigning the DNA index an important role as a biological prognostic factor in ovarian cancer.
使用流式细胞术对101例卵巢癌患者的155个样本进行了研究，以评估DNA指数和处于不同细胞周期阶段的细胞百分比。34个样本为DNA二倍体肿瘤，其余121个为DNA非整倍体肿瘤。DNA指数在不同部位以及同一患者的不同时间内都非常稳定。肿瘤分期和倍性显著相关：III期和IV期肿瘤更可能是DNA非整倍体。初次手术时残留肿瘤大小大于2 cm的患者，DNA非整倍体肿瘤的数量明显多于DNA二倍体肿瘤。DNA指数也与肿瘤的分化程度有关。细胞周期S期的细胞百分比在DNA非整倍体肿瘤和低分化肿瘤中显著高于DNA二倍体肿瘤和高分化肿瘤。使用Cox模型进行的多变量分析表明，在本研究中，DNA指数和S期细胞百分比不是独立的预后变量。在将DNA指数作为卵巢癌的生物学预后因素赋予重要作用之前，应前瞻性地收集数据。
