Elbaz Uri, Berliner Ori, Tabo Shavit, Yeshayahu Shani, Kesner Reut, Cohen-Gerassi Dana, Adler-Abramovich Lihi, Halperin-Sternfeld Michal, Aviv Moran
Ophthalmology Division, Rabin Medical Center, Petah-Tikva, 4941492, Israel.
Ophthalmology Clinic, Schneider Children's Medical Center of Israel, Petah Tikva, 4920235, Israel.
Macromol Biosci. 2025 Apr;25(4):e2400165. doi: 10.1002/mabi.202400165. Epub 2025 Feb 20.
Surgical success relies on precise tissue approximation using sutures, clips, or staples. Fibrin sealant provides a user-friendly alternative, saving time and maintaining tissue integrity. Yet, its cost and potential bioburden risk are notable drawbacks. To address these concerns, a semi-autologous fibrin sealant is produced from human cryoprecipitate and compared it to a commercial fibrin sealant. The microstructure of the semi-autologous sealant closely resembles the commercial one. Initially, the commercial sealant has superior bonding strength, however, over time, both demonstrate strong adhesive properties. Moreover, when the two sealants contain equivalent fibrinogen concentrations, they show similar bonding strength and rheological properties, including thixotropic behavior, which is essential for their application as bioadhesives. Notably, it is discovered that the mechanical properties of the adhesive are mainly governed by the fibrinogen concentration, with minimal impact of other blood components. This understanding paves the way for the development of an efficient method to boost fibrinogen in blood without extensive separation. This study indicates semi-autologous fibrin glue matches commercial sealant in adhesive properties. This may offer several advantages, such as reduced bioburden, costs, improved immunomodulation, and reduced hypersensitivity and virus transmission risks. These findings hold promising prospects for enhancing the wound healing process in various medical conditions.
手术成功依赖于使用缝线、夹子或吻合器进行精确的组织对合。纤维蛋白密封剂提供了一种用户友好的替代方法，节省时间并保持组织完整性。然而，其成本和潜在的生物负荷风险是明显的缺点。为了解决这些问题，一种半自体纤维蛋白密封剂由人冷沉淀制备而成，并将其与一种商业纤维蛋白密封剂进行比较。半自体密封剂的微观结构与商业密封剂非常相似。最初，商业密封剂具有更强的粘结强度，然而，随着时间的推移，两者都表现出很强的粘附性能。此外，当两种密封剂含有相同浓度的纤维蛋白原时，它们表现出相似的粘结强度和流变学特性，包括触变性，这对于它们作为生物粘合剂的应用至关重要。值得注意的是，发现粘合剂的机械性能主要由纤维蛋白原浓度决定，其他血液成分的影响最小。这一认识为开发一种在不进行广泛分离的情况下提高血液中纤维蛋白原的有效方法铺平了道路。这项研究表明半自体纤维蛋白胶在粘附性能上与商业密封剂相当。这可能具有几个优点，如降低生物负荷、成本、改善免疫调节以及降低超敏反应和病毒传播风险。这些发现为改善各种医疗状况下的伤口愈合过程带来了有希望的前景。
