Zheng Kaizhi, Guo Liangyong, Cao Yang, Yin Yuyang, Gao Hui, Zhang Xiaowei, Jiang Junfang, Li Jinbing, Huang Xin, Li Kui, He Sangang
Institute of Animal Husbandry and Veterinary, Zhejiang, Academy of Agricultural Sciences, Hangzhou 310021, China.
Huzhou Academy of Agricultural Sciences, Huzhou 313000, China.
Food Chem X. 2024 Oct 5;24:101871. doi: 10.1016/j.fochx.2024.101871. eCollection 2024 Dec 30.
Feeding sheep with high-concentrate diet (HCD) to shorten production cycle is a well-developed feeding strategy to increase lamb production. Here, metabolomics were performed to explore the mechanism that HCD changes lamb nutrition composition. Differential metabolites were enriched in primary bile acid biosynthesis. Significantly higher content of bile acids including taurodeoxycholic acid sodium salt (TDCA), taurochenodeoxycholic acid sodium salt (TCDCA) and taurocholic acid (TCA) was observed in lamb of HCD, while the content of lithocholic acid (LCA), cholic acid (CA), chenodeoxycholic acid (CDCA) and Chenodeoxycholic acid-3-beta-D-glucuronide (CDCA-3Gln) were higher in the controls. Furthermore, a significantly decreased content of fatty acids was observed in lamb of HCD group. Finally, primary skeletal cells treated with CA or TCA showed a significant decrease in contents of fatty acids, while TCA showed a stronger effect in decreasing fatty acid contents. Collectively, we suggest that HCD decreases lamb fatty acid contents by regulating bile acid composition.
用高浓缩日粮（HCD）喂养绵羊以缩短生产周期是一种成熟的提高羔羊产量的饲养策略。在此，进行了代谢组学研究以探索HCD改变羔羊营养成分的机制。差异代谢物在初级胆汁酸生物合成中富集。在HCD组羔羊中观察到牛磺脱氧胆酸钠盐（TDCA）、牛磺鹅脱氧胆酸钠盐（TCDCA）和牛磺胆酸（TCA）等胆汁酸含量显著更高，而对照组中石胆酸（LCA）、胆酸（CA）、鹅脱氧胆酸（CDCA）和鹅脱氧胆酸-3-β-D-葡萄糖醛酸苷（CDCA-3Gln）的含量更高。此外，在HCD组羔羊中观察到脂肪酸含量显著降低。最后，用CA或TCA处理的原代骨骼肌细胞显示脂肪酸含量显著降低，而TCA在降低脂肪酸含量方面表现出更强的作用。总体而言，我们认为HCD通过调节胆汁酸组成降低羔羊脂肪酸含量。
