Amjad Usamma, Mahajan Shreya, Choi Jiwon, Shrivastav Ritesh, Murray Raymond, Somich Abby, Coyne Olivia, Schwerdt Helen N
Department of Bioengineering, University of Pittsburgh, USA.
Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD, USA.
bioRxiv. 2025 Feb 5:2025.01.30.635139. doi: 10.1101/2025.01.30.635139.
We leveraged carbon fiber materials for creating sensors that provide dual neurochemical and electrical neural activity recording at micro-invasive (10 μm) spatial footprints proximal to recording sites, and enabling these measurements from deep brain targets of primates with conventional cranial chambers. These shaft-assisted micro-invasive probes (s-μIPs) are approximately 10 μm in diameter along the distal length (1 - 15 mm) immediately surrounding the targeted recording site. This micro-invasive portion ensures that the recording site is isolated from tissue damage induced by the wider shaft portion of the device. The shaft (150 - 165 μm in diameter) within the device stiffens the remaining length of the probe (> 100 mm), and provides compatibility with standard intracranial insertion protocols (e.g., guide tubes and chamber setups) that require a sufficiently rigid and long shaft for deep brain insertion in monkeys. The s-μIP was further expanded to provide dual-channel chemical and electrical neural activity recording with micrometer spatial resolution. Measurements of reward- and movement- related dopamine, spikes, and local field potentials were made from single and dual-channel s-μIPs implanted in task-performing monkeys. Recordings from chronically implanted s-μIPs display the capability of functional multi-modal (chemical and electrical) neural activity measurements over 1-year post-implantation from micro-invasive devices.
我们利用碳纤维材料制造传感器，这些传感器能够在靠近记录部位的微侵入性（10μm）空间范围内提供神经化学和电神经活动的双重记录，并能通过传统的颅腔对灵长类动物的深部脑靶点进行这些测量。这些轴辅助微侵入性探针（s-μIPs）在紧邻目标记录部位的远端长度（1 - 15mm）上直径约为10μm。这个微侵入部分确保记录部位与设备较宽轴部引起的组织损伤隔离开来。设备内的轴（直径150 - 165μm）使探针的其余长度（>100mm）变硬，并与标准的颅内插入方案（如导管和腔室设置）兼容，这些方案需要足够刚性和长的轴以便在猴子的深部脑进行插入。s-μIP进一步扩展以提供具有微米级空间分辨率的双通道化学和电神经活动记录。对植入执行任务猴子体内的单通道和双通道s-μIPs进行了与奖励和运动相关的多巴胺、尖峰和局部场电位的测量。长期植入的s-μIPs的记录显示出微侵入性设备在植入后1年内进行功能性多模态（化学和电）神经活动测量的能力。
