Prieto Sofia V, Orrù Bianca, Gonella Elena, Alma Alberto
Department of Agricultural, Forest and Food Sciences, University of Torino, Grugliasco, Italy.
Front Insect Sci. 2025 Feb 5;5:1520065. doi: 10.3389/finsc.2025.1520065. eCollection 2025.
Several native species in the family Pentatomidae are recorded in north-western Italy, associated with different crops. The arrival of led to a reorganization of the role of other pentatomids, some of them becoming secondary pests. Symbiont-targeted control strategies, which disrupt beneficial interactions in stink bugs, have so far been applied to . However, this approach could also be useful for controlling other pentatomid pests. Additionally, the effects of this strategy on non-target stink bug species need further investigation to assess its potential impact on agroecosystems. Here the effect of symbiont disruption was assessed for stink bugs that share host crops (, hazelnut, wheat, soybean) or the environment (especially wild areas adjacent to crops) with in north-western Italy (, , , and ). Their symbionts were identified as allied to the genus through 16S rRNA gene sequencing and also other bacteria were detected in the V4 ventricle of the midgut. Strikingly, variable symbiont infection was found across species. Laboratory tests were conducted assessing the consequences of symbiont deprivation during the first nymphal instar. Egg masses treatment with an anti-symbiont formulation affected hatching rates in and , while the mortality rates during the first instar increased in and . A correspondence between mortality induction and the alteration of symbiont infection rates was observed, with species showing the highest infection drop being the most affected by treatments. These results provide new insights into pentatomid symbionts and reveal significant variability in the response to symbiosis disruption, likely due to species-specific intensity of symbiotic interactions. The consequences of this variability are discussed.
意大利西北部记录了蝽科的几种本土物种，它们与不同的作物相关。[某种物种]的到来导致了其他蝽类角色的重新组织，其中一些变成了次要害虫。针对共生体的控制策略，即破坏椿象有益相互作用的策略，目前已应用于[某种物种]。然而，这种方法对于控制其他蝽类害虫也可能有用。此外，这种策略对非目标椿象物种的影响需要进一步研究，以评估其对农业生态系统的潜在影响。在这里，评估了在意大利西北部与[某种物种]共享寄主作物（[作物名称]、榛子、小麦、大豆）或环境（特别是作物相邻的野生区域）的椿象的共生体破坏效果（[物种名称1]、[物种名称2]、[物种名称3]、[物种名称4]和[物种名称5]）。通过16S rRNA基因测序将它们的共生体鉴定为与[属名]相关，并且在中肠的V4心室中还检测到了其他细菌。令人惊讶的是，不同物种间共生体感染情况各不相同。进行了实验室测试，评估了一龄若虫期共生体缺失的后果。用抗共生体制剂处理卵块影响了[物种名称1]和[物种名称2]的孵化率，而[物种名称3]和[物种名称4]一龄期的死亡率增加。观察到死亡率诱导与共生体感染率变化之间的对应关系，共生体感染率下降最高的物种受处理影响最大。这些结果为蝽类共生体提供了新的见解，并揭示了对共生关系破坏反应的显著变异性，这可能是由于共生相互作用的物种特异性强度所致。讨论了这种变异性的后果。
