Husseneder Claudia, Park Jong-Seok, Howells Andrea, Tikhe Chinmay V, Davis Jeffrey A
Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803 (
Environ Entomol. 2017 Feb 1;46(1):159-166. doi: 10.1093/ee/nvw112.
The redbanded stink bug, Piezodorus guildinii (Westwood) (Hemiptera: Heteroptera: Pentatomidae), is a rapidly growing pest damaging southern US agriculture. Pentatomid stink bugs are known to vector bacterial, fungal, and viral plant diseases. However, bacteria associated with redbanded stink bugs and their vector potential have not yet been assessed. In this study, we 1) cultured and identified bacteria transmitted by feeding of redbanded stink bug and 2) described bacteria from guts of redbanded stink bug individuals using next-generation sequencing of 16S rRNA genes. Nineteen bacteria transmitted by feeding of redbanded stink bug on soybean agar were isolated and identified via Sanger sequencing of near full length 16S RNA genes. The transmitted bacteria belonged to at least a dozen species in eight genera and included potential plant pathogens (Phaseolibacter flectens), plant beneficials (Bacillus atropheus), and possible insect beneficials (Acinetobacter sp. and Citrobacter farmeri). A total of 284,448 reads were captured from Illumina MiSeq sequencing of the uncultured gut bacteria community. Fifty-one putative bacteria species (74% of the estimated total species richness) were identified via matches to NCBI databases. The bacteria metagenome contained potential plant and insect pathogens (Erwinia persicina, E. rhaponici, Brenneria nigrifluens, Ralstonia picketti, and Serratia marcescens) and beneficials (Pantoea dispersa, Klebsiella oxytoca, Clostridium butyricum, and Citrobacter farmeri).
红斑蝽（Piezodorus guildinii (Westwood)，半翅目：异翅亚目：蝽科）是一种迅速蔓延的害虫，对美国南部农业造成破坏。已知蝽科椿象会传播细菌、真菌和病毒性植物病害。然而，与红斑蝽相关的细菌及其传播潜力尚未得到评估。在本研究中，我们1）培养并鉴定了红斑蝽取食传播的细菌，2）使用16S rRNA基因的下一代测序技术描述了红斑蝽个体肠道中的细菌。通过对近乎全长的16S RNA基因进行桑格测序，分离并鉴定了19种由红斑蝽在大豆琼脂上取食传播的细菌。传播的细菌至少属于8个属的12个物种，包括潜在的植物病原体（弯曲菜豆杆菌）、植物有益菌（萎缩芽孢杆菌）以及可能的昆虫有益菌（不动杆菌属和法氏柠檬酸杆菌）。从未培养的肠道细菌群落的Illumina MiSeq测序中总共捕获了284,448条读数。通过与NCBI数据库比对，鉴定出了51种假定的细菌物种（占估计总物种丰富度的74%）。细菌宏基因组包含潜在的植物和昆虫病原体（桃欧文氏菌、rhaponici欧文氏菌、黑变布伦纳氏菌、皮氏罗尔斯顿菌和粘质沙雷氏菌）以及有益菌（分散泛菌、产酸克雷伯菌、丁酸梭菌和法氏柠檬酸杆菌）。
