Rahman Md Morshedur, Siddique Naim, Gilman Md Abu Ahsan, Hasnat Soharth, Haider Md Golam, Rahman Md Morshedur, Talukder Anup Kumar, Rahman Abu Nasar Md Aminoor, Islam Tofazzal, Das Ziban Chandra, Hossain M Anwar, Hoque M Nazmul
Molecular Biology and Bioinformatics Laboratory, Department of Gynecology, Obstetrics and Reproductive Health, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, 1706, Bangladesh.
Department of Pathobiology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, 1706, Bangladesh.
Probiotics Antimicrob Proteins. 2025 Feb 20. doi: 10.1007/s12602-025-10484-8.
Pediococcus pentosaceus has the potential to be used as probiotics and biologics amid rising trends of global antimicrobial resistance (AMR) and non-communicable diseases. This study analyzed the genome of P. pentosaceus MBBL4, isolated from healthy cow milk, to assess its probiotic properties and antimicrobial efficacy. The strain was subjected to whole genome sequencing (WGS), assembly, and annotations, alongside phylogenetic and comparative genomic analyses. Additionally, carbohydrate utilization, metabolic pathways, genomic safety, and probiotic potential of MBBL4 were assessed. Its in vitro antimicrobial efficacy against mastitis pathogens was also evaluated. The WGS analysis uncovered many important probiotic traits in MBBL4. Phylogenetic analysis demonstrated a close genetic link with other 15 P. pentosaceus strains, sharing more than 99% of core genes within the pan-genome matrix. MBBL4 demonstrated extensive range of carbohydrate metabolism activity, supported by the presence of several genes encoded enzymes, including a complete elucidated lactose metabolism pathway along with 28 additional metabolic pathway modules. Notably, its genome contains regions associated with gallic acid metabolism and related genes. MBBL4 also harbored genes encoding immunity proteins like enterocin A and lactococcin, and antimicrobial compounds including penocin A, lysozymes, laccase, colicin V, and viguiepinol. Comparative analysis with other probiotic strains revealed seven novel exopolysaccharide biosynthesis proteins and one biofilm-related protein. Moreover, MBBL4 remained sensitive to 90% of the tested antibiotics and carried only a single lincosamide resistance gene (lnuA). It effectively inhibited the growth of two important bovine mastitis pathogens, Staphylococcus aureus D4C4 and Escherichia coli G1C5. These results, along with its low pathogenicity score, support the safety profile of MBBL4 and highlight its potential as bioactive natural therapeutic for mastitis and a promising probiotic candidate.
在全球抗菌药物耐药性（AMR）和非传染性疾病呈上升趋势的背景下，戊糖片球菌有潜力用作益生菌和生物制品。本研究分析了从健康牛奶中分离出的戊糖片球菌MBBL4的基因组，以评估其益生菌特性和抗菌效果。该菌株进行了全基因组测序（WGS）、组装和注释，同时进行了系统发育和比较基因组分析。此外，还评估了MBBL4的碳水化合物利用、代谢途径、基因组安全性和益生菌潜力。还评估了其对乳腺炎病原体的体外抗菌效果。WGS分析揭示了MBBL4中许多重要的益生菌特性。系统发育分析表明，它与其他15株戊糖片球菌菌株有密切的遗传联系，在泛基因组矩阵中共享超过99%的核心基因。MBBL4表现出广泛的碳水化合物代谢活性，这由几个编码酶的基因的存在所支持，包括一条完整阐明的乳糖代谢途径以及另外28个代谢途径模块。值得注意的是，其基因组包含与没食子酸代谢相关的区域和相关基因。MBBL4还含有编码免疫蛋白如肠球菌素A和乳球菌素的基因，以及抗菌化合物，包括青霉素A、溶菌酶、漆酶、大肠杆菌素V和维吉菌素。与其他益生菌菌株的比较分析揭示了7种新型胞外多糖生物合成蛋白和1种生物膜相关蛋白。此外，MBBL4对90%的测试抗生素仍敏感，仅携带一个林可酰胺抗性基因（lnuA）。它有效抑制了两种重要的牛乳腺炎病原体金黄色葡萄球菌D4C4和大肠杆菌G1C5的生长。这些结果，连同其低致病性评分，支持了MBBL4的安全性，并突出了其作为乳腺炎生物活性天然治疗剂的潜力以及作为有前途的益生菌候选者的潜力。
