Xu Jiahao, Zou Dongzhe, Dong Enyu, Jiang Xingyu, Xu Shuo, Xiao Yusheng, Gong Wensheng, Xu Yini, Zeng Zenan, Deng Kefurong, Li Yachao, Xu Xianghui
Department of Pharmacy, College of Biology, Hunan University, Changsha, Hunan 410082, China.
State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha, Hunan 410082, China.
ACS Biomater Sci Eng. 2025 Jul 14;11(7):4166-4176. doi: 10.1021/acsbiomaterials.4c02045. Epub 2025 Feb 20.
The rising incidence of bacterial infections poses a significant challenge to global public health. The development of safe and effective antibacterial treatment strategies is an urgent need in the field of biomedicine. In this work, we developed a smart nanoparticle-hydrogel platform to address bacterial infections in wounds. Rifampicin-loaded chitosan-functionalized nanoparticles (R-CNP) could break bacterial barriers and enhance antibiotic internalization. R-CNP reduced the minimum inhibitory concentration of rifampicin against and greatly enhanced the bactericidal effect of rifampicin. Furthermore, R-CNP was incorporated into thermosensitive hydrogels (HG) to construct HG(R-CNP) for enhanced antibiotic accumulation and wound protection. In the mouse model with a bacterial-infected wound, treatment with R-CNP reduced the bacterial content by 98.5% as compared to treatment with free rifampicin. Therefore, this smart nanoparticle-hydrogel platform constructed by FDA-approved or natural polymers, offers significant therapeutic efficacy on bacterial-infected wounds, showing great promise for clinical translation.
细菌感染发病率的上升对全球公共卫生构成了重大挑战。开发安全有效的抗菌治疗策略是生物医学领域的迫切需求。在这项工作中，我们开发了一种智能纳米颗粒-水凝胶平台来解决伤口中的细菌感染问题。负载利福平的壳聚糖功能化纳米颗粒（R-CNP）可以突破细菌屏障并增强抗生素的内化。R-CNP降低了利福平对[具体细菌]的最低抑菌浓度，并大大增强了利福平的杀菌效果。此外，将R-CNP掺入热敏水凝胶（HG）中以构建HG(R-CNP)，以增强抗生素积累和伤口保护。在细菌感染伤口的小鼠模型中，与游离利福平治疗相比，R-CNP治疗使细菌含量降低了98.5%。因此，这个由FDA批准的或天然聚合物构建的智能纳米颗粒-水凝胶平台，对细菌感染伤口具有显著的治疗效果，显示出巨大的临床转化前景。
