铜绿假单胞菌临床菌株的毒力特征及基因型分布。

The virulence trait and genotype distribution amongst the Pseudomonas aeruginosa clinical strains.

作者信息

Wang Xiaohuan, Gao Kaijing, Pan Baishen, Wang Beili, Song Yuanlin, Guo Wei

机构信息

Department of Laboratory Medicine, Zhongshan Hospital, Fudan University, 111 Yi Xue Yuan Road, Shanghai, 200032, China.

Shanghai Key Laboratory of Lung Inflammation and Injury, Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.

出版信息

BMC Microbiol. 2025 Feb 20;25(1):82. doi: 10.1186/s12866-025-03754-6.

DOI:10.1186/s12866-025-03754-6

PMID:39979804

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11841163/

Abstract

BACKGROUND

Pseudomonas aeruginosa is notorious for its complex virulence system and rapid adaptive drug resistance. This study aimed to compare the prevalence and genotype distribution of virulence genes in multidrug-sensitive and multidrug-resistant clinical strains of Pseudomonas aeruginosa. It is possible to better understand the genetic characteristics of Pseudomonas aeruginosa and carry out effective treatment and prevention measures.

METHODS

The genes phzS, aprA, plcH, toxA, pilA and exoU were detected amongst 184 clinical strains, whose cytotoxicity and biofilm formation ability were evaluated as well. Phenotypic screening for drug susceptibility was conducted by standard antimicrobial susceptibility test and interpreted according to standards established by CLSI.

RESULTS

A total of 94 multidrug-sensitive and 90 multidrug-resistant isolates were included in this study. Statistically significant relationship was observed in the frequency of the toxA (p = 0.002) and plcH (p = 0.001) genes between multidrug-resistant and multidrug-sensitive strains. Moreover, thirteen genotypes were observed in multidrug-sensitive strains, and seven of them were included in multidrug-resistant groups. There was statistically significant correlation found between the presence of genotype IV (p = 0.001) and genotype VII (p = 0.001) in two subgroups. Additionally, It was found that genotype III isolates exhibited most obvious cytotoxicity, and multidrug-resistant isolates of genotype III showed the most significant cytotoxicity. Moreover, the strains of strong biofilm-formation accounted for a relatively high proportion in genotype III and VI groups.

CONCLUSION

These virulence genes could form abundant genotype varieties, whose overall number is greater in multi-sensitive strains. In addition, particular genotypes were characteristically distributed and exhibited different cytotoxicity and biofilm-formation abilities.

摘要

背景

铜绿假单胞菌因其复杂的毒力系统和快速的适应性耐药而臭名昭著。本研究旨在比较多药敏感和多药耐药的铜绿假单胞菌临床菌株中毒力基因的流行情况和基因型分布。以便更好地了解铜绿假单胞菌的遗传特征，并采取有效的治疗和预防措施。

方法

在184株临床菌株中检测phzS、aprA、plcH、toxA、pilA和exoU基因，并评估其细胞毒性和生物膜形成能力。通过标准抗菌药物敏感性试验进行药物敏感性的表型筛选，并根据CLSI制定的标准进行解读。

结果

本研究共纳入94株多药敏感菌株和90株多药耐药菌株。多药耐药菌株和多药敏感菌株之间，toxA基因（p = 0.002）和plcH基因（p = 0.001）的频率存在统计学显著差异。此外，在多药敏感菌株中观察到13种基因型，其中7种包含在多药耐药组中。在两个亚组中，基因型IV（p = 0.001）和基因型VII（p = 0.001）的存在之间存在统计学显著相关性。此外，发现基因型III分离株表现出最明显的细胞毒性，基因型III的多药耐药分离株表现出最显著的细胞毒性。此外，在基因型III和VI组中，强生物膜形成菌株占比较高。