Shinohara Y, Baba S, Kasuya Y
J Chromatogr. 1985 Mar 22;338(2):281-8. doi: 10.1016/0378-4347(85)80099-2.
A method for the quantitative estimation of methyltestosterone and methyltestosterone-d3 in biological fluids has been developed using gas chromatography-mass spectrometry-selected-ion monitoring. Methyltestosterone-d6 was used as an internal standard. Methyltestosterone and methyltestosterone-d3 in serum were determined based on the peak height ratios of the molecular ions of methyltestosterone, methyltestosterone-d3 and methyltestosterone-d6. Sensitivity, specificity, precision, accuracy and reproducibility of the present method were demonstrated to be satisfactory for application to pharmacokinetic and bioavailability studies.
