风疹IgG和IgM抗体酶联免疫吸附测定试剂盒的评估
An evaluation of ELISA kits for rubella IgG- and IgM-antibodies.
作者信息
Paris-Hamelin A, Fustec-Ibarboure S
出版信息
J Virol Methods. 1985 Apr;10(4):355-61. doi: 10.1016/0166-0934(85)90053-9.
Two ELISA kits for rubella IgG- and IgM-antibodies, respectively, were compared with a haemagglutination inhibition (HAI) test (after ultracentrifugation of the sample if IgM was to be detected). When screening 535 samples from pregnant women, 99.6% agreement was found between the IgG-ELISA and HAI; one true IgM-positive was encountered in this group with the IgM-ELISA. An agreement of 95.5% was found between the IgM-ELISA and HAI after ultracentrifugation. In at least some of the discrepant samples the IgM-ELISA may be assumed to have given the correct answer.
分别对两种用于检测风疹IgG和IgM抗体的酶联免疫吸附测定（ELISA）试剂盒与血凝抑制（HAI）试验进行了比较（如果要检测IgM，则在对样本进行超速离心后进行HAI试验）。在筛查535名孕妇的样本时，IgG-ELISA与HAI之间的一致性为99.6%；在该组中，IgM-ELISA检测到1例假阳性IgM。超速离心后，IgM-ELISA与HAI之间的一致性为95.5%。在至少一些有差异的样本中，可以认为IgM-ELISA给出了正确结果。
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