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一种用于评估风疹特异性IgM抗体的新型酶联免疫吸附测定（ELISA）方法

Evaluation of a new ELISA method for estimating rubella-specific IgM antibody.

作者信息

Cubie H A

出版信息

J Infect. 1983 Sep;7(2):144-50. doi: 10.1016/s0163-4453(83)90590-x.

DOI:10.1016/s0163-4453(83)90590-x
PMID:6358371
Abstract

Results of using a new enzyme-linked immunosorbent assay (ELISA) kit for detecting rubella IgM antibodies were compared with those obtained by fractionating samples of serum on sucrose density gradients followed by rubella haemagglutination inhibition tests on the fractions. There was close agreement between the two methods. A major advantage of the ELISA method was the ability to complete tests within one day.

摘要

使用一种新型酶联免疫吸附测定（ELISA）试剂盒检测风疹IgM抗体的结果，与通过在蔗糖密度梯度上分离血清样本，然后对各组分进行风疹血凝抑制试验所获得的结果进行了比较。两种方法结果高度一致。ELISA方法的一个主要优点是能够在一天内完成检测。

相似文献

1
Evaluation of a new ELISA method for estimating rubella-specific IgM antibody.一种用于评估风疹特异性IgM抗体的新型酶联免疫吸附测定（ELISA）方法
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[Studies on the management of pregnant women having high hemagglutination inhibition (HI) antibody titer of rubella virus in the first trimester examination of rubella virus antibody in the pregnant women having high HI titer by different methods and prognosis of their infants after birth].[关于妊娠早期风疹病毒血凝抑制（HI）抗体效价高的孕妇风疹病毒抗体检测不同方法的管理及出生后婴儿预后的研究]
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Comparison of the performance and reproducibility of various serological methods and diagnostic kits for the detection of rubella antibodies.用于检测风疹抗体的各种血清学方法和诊断试剂盒的性能及可重复性比较。
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引用本文的文献

1
Clinical evaluation of the sensitivity and specificity of a commercially available enzyme immunoassay for detection of rubella virus-specific immunoglobulin M.一种用于检测风疹病毒特异性免疫球蛋白M的市售酶免疫测定法的敏感性和特异性的临床评估。
J Clin Microbiol. 1984 Sep;20(3):400-4. doi: 10.1128/jcm.20.3.400-404.1984.
2
Comparison of five different methods of rubella IgM antibody testing.五种不同风疹IgM抗体检测方法的比较
J Clin Pathol. 1985 Feb;38(2):203-7. doi: 10.1136/jcp.38.2.203.
3
Development of a highly specific and sensitive rubella immunoglobulin M antibody capture enzyme immunoassay that uses enzyme-labeled antigen.
一种使用酶标记抗原的高度特异性和敏感性风疹免疫球蛋白M抗体捕获酶免疫测定法的研发。
J Clin Microbiol. 1990 Apr;28(4):719-23. doi: 10.1128/jcm.28.4.719-723.1990.