Arendt J, Bojkowski C, Franey C, Wright J, Marks V
J Clin Endocrinol Metab. 1985 Jun;60(6):1166-73. doi: 10.1210/jcem-60-6-1166.
An assessment of the rhythmic characteristics of melatonin secretion in man and other species requires the determination of 24-h secretion profiles. Measurement of a major excreted metabolite would allow noninvasive study of pineal function, applicable in particular to pediatric and long term circadian rhythm studies. This report describes a simple and rapid RIA for 6-hydroxymelatonin sulfate in human plasma and urine. Physiological studies revealed that both plasma and urinary levels of 6-hydroxymelatonin sulfate were closely related to plasma melatonin, and that the urinary 24-h rhythm was abolished by the beta 1-adrenergic anagonist atenolol.
评估人类和其他物种褪黑素分泌的节律特征需要测定24小时分泌曲线。对一种主要排泄代谢物的测量将有助于对松果体功能进行无创研究，尤其适用于儿科和长期昼夜节律研究。本报告描述了一种用于测定人血浆和尿液中硫酸6-羟基褪黑素的简单快速放射免疫分析法。生理学研究表明，硫酸6-羟基褪黑素的血浆和尿液水平均与血浆褪黑素密切相关，并且β1-肾上腺素能拮抗剂阿替洛尔可消除尿液中的24小时节律。
