Hart J P, Shearer M J, Klenerman L, Catterall A, Reeve J, Sambrook P N, Dodds R A, Bitensky L, Chayen J
J Clin Endocrinol Metab. 1985 Jun;60(6):1268-9. doi: 10.1210/jcem-60-6-1268.
If gamma-carboxylation, by the vitamin K1 - cycle, of glutamate residues of bone-matrix peptides is essential for the formation of bone, the circulating levels of this vitamin might indicate the potential efficiency of this process. Methods involving HPLC with electrochemical detection have very recently been developed for assaying the low levels of vitamin K1 that occur in normal plasma. Using such methods, we found that the circulating levels of vitamin K1 in osteoporotic patients (who had sustained either spinal crush-fractures or fractures of the neck of the femur) were significantly lower than those of age-matched control subjects.
如果通过维生素K1循环对骨基质肽的谷氨酸残基进行γ-羧化作用对骨的形成至关重要，那么这种维生素的循环水平可能表明该过程的潜在效率。最近已经开发出了采用电化学检测的高效液相色谱法来测定正常血浆中低水平的维生素K1。使用这些方法，我们发现骨质疏松症患者（患有脊椎压缩性骨折或股骨颈骨折）的维生素K1循环水平显著低于年龄匹配的对照受试者。
