Deng Yaqin, Zhu Zhikai, Chen Jian, Kuang Liuhui, Yan Tao, Li Lin, Wu Dezhi, Gao Fei
College of Agronomy, Hunan Agricultural University, Changsha, China; Yuelushan Laboratory, Changsha, 410128, China.
College of Agronomy, Hunan Agricultural University, Changsha, China; Yuelushan Laboratory, Changsha, 410128, China.
Plant Physiol Biochem. 2025 Apr;221:109668. doi: 10.1016/j.plaphy.2025.109668. Epub 2025 Feb 18.
Heat stress is one of the most critical environmental factors impacting rice cultivation, driven by the rising global temperatures. Therefore, understanding the differences in molecular mechanisms of heat stress tolerance between rice cultivars, particularly indica and japonica, is crucial for developing heat-tolerant varieties. In this study, high throughput RNA-sequencing technology was utilized to explore the transcriptomic changes in the root tissues of two rice varieties, 93-11 (indica) and ZH11 (japonica) under heat stress and during recovery. Differentially Expressed Genes (DEGs) analysis revealed that ZH11 had 14,719 DEGs after the two-day heat treatment, and 10,178 DEGs during the recovery process. In contrast, 93-11 had a lower number of DEGs than ZH11 in both the heat treatment and recovery phases, with 12,433 DEGs and 5986 DEGs, respectively. The GO and KEGG analyses showed that the two rice varieties shared several enriched pathways in response to heat stress. However, each cultivar also had its own uniquely enriched pathways, reflecting distinct responses to heat stress in ZH11 and 93-11. In addition, WGCNA analysis highlighted that the OsMAPK3 is novel hub gene in response to heat stress in rice. Knockout of OsMAPK3 compromises rice heat stress tolerance. These results provide new insights into the differences in molecular mechanisms of heat stress response in roots between indica and japonica rice cultivars, offering valuable targets for genetic improvement and breeding programs aimed at developing heat-tolerant rice varieties.
热胁迫是影响水稻种植的最关键环境因素之一，这是由全球气温上升所驱动的。因此，了解水稻品种特别是籼稻和粳稻之间耐热胁迫分子机制的差异，对于培育耐热品种至关重要。在本研究中，利用高通量RNA测序技术探究了两个水稻品种93-11（籼稻）和ZH11（粳稻）在热胁迫及恢复过程中根组织的转录组变化。差异表达基因（DEG）分析显示，经过两天热处理后，ZH11有14719个差异表达基因，恢复过程中有10178个差异表达基因。相比之下，93-11在热处理和恢复阶段的差异表达基因数量均低于ZH11，分别为12433个和5986个。基因本体（GO）和京都基因与基因组百科全书（KEGG）分析表明，这两个水稻品种在应对热胁迫时共有几个富集途径。然而，每个品种也有其独特的富集途径，反映了ZH11和93-11对热胁迫的不同反应。此外，加权基因共表达网络分析（WGCNA）强调，OsMAPK3是水稻应对热胁迫的一个新的核心基因。敲除OsMAPK3会损害水稻的耐热胁迫能力。这些结果为籼稻和粳稻品种根中热胁迫反应分子机制的差异提供了新的见解，为旨在培育耐热水稻品种的遗传改良和育种计划提供了有价值的靶点。
